Various UVB doses affect change of raf kinase inhibitor protein, nitric oxide and proliferation in keratinocytes

UVB is a potent modulator of cell growth and differentiation in the skin. The UVB irradiation has been used in treating hyperproliferative dermatoses. Otherwise, UVB radiation is also the major risk factor for developing skin cancer. Nitric oxide (NO) has been suggested to be a physiological modulat...

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Bibliographic Details
Published in:Toxicology in vitro 2017-08, Vol.42, p.101-104
Main Authors: Wu, Chieh-Shan, Tsao, Der-An, Yu, Hsin-Su, Chien, Ching-Yen, Chang, Huoy-Rou
Format: Article
Language:English
Subjects:
Cell growth
Cell proliferation
Cell Proliferation - radiation effects
Cells, Cultured
Differentiation
Enzyme inhibitors
Health risks
Humans
Hyperplasia
Inducible nitric oxide synthase (iNOS)
Irradiation
Keratinocyte
Keratinocytes
Keratinocytes - metabolism
Keratinocytes - radiation effects
Kinases
Nitric oxide
Nitric oxide (NO)
Nitric Oxide - metabolism
Nitric Oxide Synthase Type II - metabolism
Nitric-oxide synthase
Phosphatidylethanolamine Binding Protein - metabolism
Proliferation
Proteins
Radiation
Raf kinase inhibitor protein (RKIP)
Raf protein
Ribonucleotide reductase
Risk analysis
Risk factors
Skin cancer
Ultraviolet radiation
Ultraviolet Rays
UVB
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Summary:UVB is a potent modulator of cell growth and differentiation in the skin. The UVB irradiation has been used in treating hyperproliferative dermatoses. Otherwise, UVB radiation is also the major risk factor for developing skin cancer. Nitric oxide (NO) has been suggested to be a physiological modulator of cell proliferation. Raf-1 kinase inhibitory protein (RKIP) was involved in cell growth, transformation, and differentiation. The purpose of this study was to search for the possible cause of UVB-inhibited hyperplasia and UVB-resulted hyperproliferation. We evaluated various UVB dose whether affect the expression of RKIP, iNOS, NO and proliferation in keratinocyte. Normal human keratinocytes were treated with UVB dose of 40mJ/cm2, 80mJ/cm2, 120mJ/cm2, 160mJ/cm2 and 0mJ/cm2 (control group) respectively. The results showed that RKIP, iNOS and NO of keratinocytes with doses of 40mJ/cm2 and 80mJ/cm2 UVB treatment significantly higher than control group (P
ISSN:0887-2333
1879-3177
DOI:10.1016/j.tiv.2017.04.011