A facile horseradish peroxidase electrochemical biosensor with surface molecular imprinting based on polyaniline nanotubes

• Published in: Journal of electroanalytical chemistry (Lausanne, Switzerland) Switzerland), 2018-05, Vol.817, p.184-194
• Main Authors: Wang, Qin, Xue, Rui, Guo, Hao, Wei, Yuli, Yang, Wu
• Format: Article
• Language: English
• Subjects: Biosensors; Catalytic activity; Electrical measurement; Electrochemical imprinted biosensor; Electron transfer; Fourier transforms; Horseradish peroxidase; Hydrogen peroxide; Molecular imprinting; Nanotubes; Peroxidase; Polyaniline nanotubes; Polyanilines; Self-assembly; Urine; Voltammetry
• ISSN: 1572-6657; 1873-2569
• DOI: 10.1016/j.jelechem.2018.04.013

A green, highly sensitive and selective electrochemical imprinted biosensor for horseradish peroxidase (HRP) was designed and prepared based on the combination of polyaniline nanotubes (PANTs) and self-assembly surface molecular imprinting technique. PANI nanotubes, as supporting material, could effectively enhance imprinting efficiency and the electrode conductivity and facilitate electron transfer. The imprinted biosensor was characterized by electrochemical methods, Fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM). It showed a very highly electrocatalytical activity for reduction of hydrogen peroxide and could be used in amperometric determination of H2O2 in the ranges of 0–10 μM and 10–90 μM with a detection limit of 0.01 μM (S / N = 3). The imprinted sensor showed still an excellent selectivity for HRP and was successfully employed to detect different concentration levels of HRP from 1.0 × 10−9 to 0.10 mg/mL with a detection limit of 3.56 × 10−10 mg/mL(8.1fM) (S / N = 3) with Fe(CN)63/4− probe by differential pulse voltammetry(DPV). The developed method was successfully used in determination of H2O2 in human urine sample. A HRP surface imprinting electrochemical sensor based PANTs film for sensitive and selective determination of hydrogen peroxide and horseradish peroxidase. [Display omitted] •A new and simple electrochemical imprinting film biosensor for H2O2 and HRP was prepared.•The prepared sensor showed a good electrochemical response, high sensitivity and selectivity for HRP determination.•The sensor was also used to detect H2O2 in biological sample successfully.