Phenotypic and genotypic characterization of mutants of the virginiamycin producing strain 899 and its relatedness to the type strain of Streptomyces virginiae

A representative set of 19 mutants, with a known genealogy, of the virginiamycin producing strain Streptomyces virginiae 899 was investigated phenotypically and genotypically. Colour of the aerial and substrate mycelium were very variable both among spontaneous variants and those obtained after indu...

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Bibliographic Details
Published in:Systematic and applied microbiology 2005-01, Vol.28 (1), p.77-84
Main Authors: Lanoot, B., Vancanneyt, M., Hoste, B., Cnockaert, M.C., Piecq, M., Gosselé, F., Swings, J.
Format: Article
Language:English
Subjects:
AFLP
Bacteriology
Base Composition
Biological and medical sciences
BOX-PCR
DNA Fingerprinting
DNA, Bacterial - analysis
DNA, Bacterial - genetics
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
Genetics
Microbiology
Mutants
Nucleic Acid Hybridization
Phenotype
Pigments, Biological
Point Mutation - genetics
Point Mutation - physiology
Polymorphism, Genetic
Streptomyces - classification
Streptomyces - genetics
Streptomyces - physiology
Streptomyces virginiae
Systematics
Virginiamycin - biosynthesis
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Summary:A representative set of 19 mutants, with a known genealogy, of the virginiamycin producing strain Streptomyces virginiae 899 was investigated phenotypically and genotypically. Colour of the aerial and substrate mycelium were very variable both among spontaneous variants and those obtained after induced mutagenesis. At genotypic level, all mutants showed nearly identical BOX patterns, not reflecting the phenotypic heterogeneity observed. More than 40 years of forced mutational pressure did not cause huge chromosomal distortions but was most likely limited to base substitutions. The specie s S. virginiae, including besides producers of virginiamycin the type strain and non-type strains producing other bioactive compounds , is genomically heterogeneous on the basis of BOX-PCR fingerprinting and DNA–DNA hybridizations. The virginiamycin producing strain 899 does not belong to the species S. virginiae despite its phenotypic similarity to the latter.
ISSN:0723-2020
1618-0984
DOI:10.1016/j.syapm.2004.09.006