N-methylpyrrolidone exfoliated graphene as sensitive electrochemical sensing platform for 10-Hydroxycamptothecine

10-Hydroxycamptothecine (10-HCPT), a natural alkaloid, is widely used in clinical practice because of its significant anti-cancer activity and small toxic side effects. In this work, graphene nanosheets (GNs) were prepared by ultrasonic exfoliation of graphite using N-methylpyrrolidone (NMP) as the...

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Bibliographic Details
Published in:Journal of electroanalytical chemistry (Lausanne, Switzerland) Switzerland), 2018-06, Vol.818, p.210-215
Main Authors: Ye, Xiaoxia, Yang, Xiaofeng, Wei, Piaopiao, Wu, Kangbing
Format: Article
Language:English
Subjects:
10-Hydroxycamptothecine
Anticancer properties
Colorectal cancer
Electrochemical determination
Exfoliation
Glassy carbon
Graphene
Graphite
Nanostructured materials
Oxidation
Scanning electron microscopy
Side effects
Solvent exfoliation
Transmission electron microscopy
Urine
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Summary:10-Hydroxycamptothecine (10-HCPT), a natural alkaloid, is widely used in clinical practice because of its significant anti-cancer activity and small toxic side effects. In this work, graphene nanosheets (GNs) were prepared by ultrasonic exfoliation of graphite using N-methylpyrrolidone (NMP) as the solvent. The prepared GNs were characterized using transmission electron microscopy and scanning electron microscopy, and then used to construct a novel sensing platform for 10-HCPT. The electrochemical behaviors of 10-HCPT on the glassy carbon electrode (GCE) and GNs modified GCE (GNs/GCE) were studied. Compared with bare GCE, the prepared GNs/GCE increased the oxidation signal of 10-HCPT significantly, owing to larger active area of GNs/GCE and thus higher accumulation efficiency of 10-HCPT. Utilizing the signal amplification strategy of GNs, a highly-sensitive electrochemical method was established for the detection of 10-HCPT. The linear range was from 10.0 to 250.0 μg L−1, and the detection limit was 3.21 μg L−1 (8.81 nM). It was used to detect 10-HCPT in urine samples, and satisfactory results were obtained.
ISSN:1572-6657
1873-2569
DOI:10.1016/j.jelechem.2018.04.048