Sparse reconstructions of overlapping three-dimensional point spread functions using overcomplete dictionaries

When a single molecule is detected in a wide-field microscope, the image approximates the point spread function of the system. However, as the distribution of molecules becomes denser and their images begin to coincide, existing solutions to determine the number of molecules present and their precis...

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Bibliographic Details
Published in:arXiv.org 2013-08
Main Authors: Barsic, Anthony, Grover, Ginni, Piestun, Rafael
Format: Article
Language:English
Subjects:
Digital techniques
Dimensional tolerances
Image detection
Point spread functions
Online Access:Get full text
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Summary:When a single molecule is detected in a wide-field microscope, the image approximates the point spread function of the system. However, as the distribution of molecules becomes denser and their images begin to coincide, existing solutions to determine the number of molecules present and their precise three-dimensional locations can tolerate little to no overlap. A solution to this problem involving matched optical and digital techniques, as here proposed, is critical to increase the allowable labeling density and to accelerate single-molecule localization microscopy.
ISSN:2331-8422