Mapping cellular magnesium using X-ray microfluorescence and atomic force microscopy

Magnesium is the most abundant intracellular divalent cation. We present an innovative experimental approach to localizing intracellular magnesium that combines elemental and morphological information from individual cells with high-resolution spatial information. Integration of information from sca...

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Bibliographic Details
Published in:arXiv.org 2010-07
Main Authors: Lagomarsino, Stefano, Iotti, Stefano, Farruggia, Giovanna, Cedola, Alessia, Trapani, Valentina, Fratini, Michela, Bukreeva, Inna, Notargiacomo, Andrea, Mastrototaro, Lucia, McNulty, Ian, Vogt, Stefan, Legnini, Daniel, Kim, Sangsoo, Maier, Jeanette A M, Wolf, Federica I
Format: Article
Language:English
Subjects:
Absorptivity
Atomic force microscopy
Dehydration
Fluorescence
Magnesium
Mapping
Microscopy
Spatial data
X ray microscopy
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Summary:Magnesium is the most abundant intracellular divalent cation. We present an innovative experimental approach to localizing intracellular magnesium that combines elemental and morphological information from individual cells with high-resolution spatial information. Integration of information from scanning fluorescence X-ray microscopy with information from atomic force microscopy was used to generate a magnesium concentration map and to determine the X-ray linear absorption coefficient map within a whole dehydrated mammary epithelial cell.
ISSN:2331-8422