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Mapping cellular magnesium using X-ray microfluorescence and atomic force microscopy
Magnesium is the most abundant intracellular divalent cation. We present an innovative experimental approach to localizing intracellular magnesium that combines elemental and morphological information from individual cells with high-resolution spatial information. Integration of information from sca...
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| Published in: | arXiv.org 2010-07 |
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| Main Authors: | , , , , , , , , , , , , , , |
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| creator | Lagomarsino, Stefano Iotti, Stefano Farruggia, Giovanna Cedola, Alessia Trapani, Valentina Fratini, Michela Bukreeva, Inna Notargiacomo, Andrea Mastrototaro, Lucia McNulty, Ian Vogt, Stefan Legnini, Daniel Kim, Sangsoo Maier, Jeanette A M Wolf, Federica I |
| description | Magnesium is the most abundant intracellular divalent cation. We present an innovative experimental approach to localizing intracellular magnesium that combines elemental and morphological information from individual cells with high-resolution spatial information. Integration of information from scanning fluorescence X-ray microscopy with information from atomic force microscopy was used to generate a magnesium concentration map and to determine the X-ray linear absorption coefficient map within a whole dehydrated mammary epithelial cell. |
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We present an innovative experimental approach to localizing intracellular magnesium that combines elemental and morphological information from individual cells with high-resolution spatial information. Integration of information from scanning fluorescence X-ray microscopy with information from atomic force microscopy was used to generate a magnesium concentration map and to determine the X-ray linear absorption coefficient map within a whole dehydrated mammary epithelial cell.</description><identifier>EISSN: 2331-8422</identifier><language>eng</language><publisher>Ithaca: Cornell University Library, arXiv.org</publisher><subject>Absorptivity ; Atomic force microscopy ; Dehydration ; Fluorescence ; Magnesium ; Mapping ; Microscopy ; Spatial data ; X ray microscopy</subject><ispartof>arXiv.org, 2010-07</ispartof><rights>2010. This work is published under http://arxiv.org/licenses/nonexclusive-distrib/1.0/ (the “License”). 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| identifier | EISSN: 2331-8422 |
| ispartof | arXiv.org, 2010-07 |
| issn | 2331-8422 |
| language | eng |
| recordid | cdi_proquest_journals_2087131638 |
| source | Publicly Available Content Database |
| subjects | Absorptivity Atomic force microscopy Dehydration Fluorescence Magnesium Mapping Microscopy Spatial data X ray microscopy |
| title | Mapping cellular magnesium using X-ray microfluorescence and atomic force microscopy |
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