ATR-FTIR spectroscopy detects alterations induced by organotin(IV) carboxylates in MCF-7 cells at sub-cytotoxic/-genotoxic concentrations

The environmental impact of metal complexes such as organotin(IV) compounds is of increasing concern. Genotoxic effects of organotin(IV) compounds (0.01 microg/ml, 0.1 microg/ml or 1.0 microg/ml) were measured using the alkaline single-cell gel electrophoresis (comet) assay to measure DNA single-str...

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Bibliographic Details
Published in:arXiv.org 2009-01
Main Authors: Ahmad, Muhammad S, Mirza, Bushra, Hussain, Mukhtiar, Hanif, Muhammad, Ali, Saqib, Walsh, Michael J, Martin, Francis L
Format: Article
Language:English
Subjects:
Bioassays
Carboxylates
Coordination compounds
Damage detection
Deoxyribonucleic acid
DNA
Electrophoresis
Environmental impact
Fourier transforms
Genotoxicity
Infrared reflection
Infrared signatures
Infrared spectra
Infrared spectroscopy
Interrogation
Spectrum analysis
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Summary:The environmental impact of metal complexes such as organotin(IV) compounds is of increasing concern. Genotoxic effects of organotin(IV) compounds (0.01 microg/ml, 0.1 microg/ml or 1.0 microg/ml) were measured using the alkaline single-cell gel electrophoresis (comet) assay to measure DNA single-strand breaks (SSBs) and the cytokinesis-block micronucleus (CBMN) assay to determine micronucleus formation. Biochemical-cell signatures were also ascertained using attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy. In the comet assay, organotin(IV) carboxylates induced significantly-elevated levels of DNA SSBs. Elevated micronucleus-forming activities were also observed. Following interrogation using ATR-FTIR spectroscopy, infrared spectra in the biomolecular range (900 cm-1 - 1800 cm-1) derived from orga...
ISSN:2331-8422
DOI:10.48550/arxiv.0901.2039