In-vitro and in-vivo therapeutic study the effect of Ca-EDTA against Salmonella typhimurium infection in experimental local rabbits

[...]Ca-EDTA can act synergistically with gentamicin to treat the rabbits with salmonellosis by reducing length and frequency of treatment. [...]of this disruption process, the membrane become more permeable to other agents (9,10). Group 1 : five rabbits were used as negative control which drenched...

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Bibliographic Details
Published in:Journal of pharmaceutical sciences and research 2018-07, Vol.10 (7), p.1765-1767
Main Authors: Hasan, Mustafa Salah, Abdulga, Ayman Barzan, Hussein, Mohammed Ali, Owain, Maher Saber, Aboud, Qusay Mohammed, Al-Zobaie, Anmar J
Format: Article
Language:English
Subjects:
Antibiotics
Antimicrobial agents
Bacteria
Biofilms
Catheters
Chemotherapy
Drug dosages
Drug resistance
Infections
Laboratories
Medical research
Potassium
Preventive medicine
Salmonella
Vaccines
Veterinary colleges
Veterinary medicine
Zoonoses
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Summary:[...]Ca-EDTA can act synergistically with gentamicin to treat the rabbits with salmonellosis by reducing length and frequency of treatment. [...]of this disruption process, the membrane become more permeable to other agents (9,10). Group 1 : five rabbits were used as negative control which drenched orally by plastic syringe with normal saline at dose 5 ml, Group 2: five rabbits were used as infected group which drenched 5 ml suspension which have (5·109 CFU) of Salmonella typhimurium, Group 3 : five rabbits were drenched 5 ml suspension having (5·109 CFU) of Salmonella typhimurium then treated with single dose of gentamicin (Vapco, Jordan) alone at 0.05ml/kg (5mg/ml) orally (according to manufacturer) after presence of signs (24hrs. post infection), Group 4 : five rabbits were drenched 5 ml suspension containing (5·109) CFU of S. typhimurium then treated with single dose of Ca-EDTA (GLS company, USA) alone at 40mg/kg orally (according to manufacturer) after presence of signs (24hrs. post infection), Group 5 : five rabbits were drenched 5 ml suspension containing (5·109) CFU of S. typhimurium then treated with combination of single dose of gentamicin at 0.05ml/kg (5mg/ml) orally and CaEDTA 40mg/kg orally after presence of signs (24hrs. post infection). (19) who documented the capability of Ca-EDTA chelators to eradicate some bacteria, they experimented Gentamicin-EDTA invivo in rats against Pseudomonas aeruginosa, Escherichia coli, Staphylococcus epidermidis, and Staphylococcus aureus, also they proved that the use of EDTA (30 mg/ml) as a supplement to the gentamicin (5 mg/ml) antibiotic lock solution were completely eradicated Gram-positive and Gram-negative bacteria.
ISSN:0975-1459