New Procedure To Readily Investigate Lactase Enzymatic Activity Using Fehling’s Reagent

Determination of enzymatic activity is a relevant experiment in teaching chemistry and biochemistry to high school and college students. A method for measuring the kinetic parameters of lactose cleavage by the enzyme lactase is described. This enzyme catalyzes the hydrolysis of lactose to galactose...

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Bibliographic Details
Published in:Journal of chemical education 2018-07, Vol.95 (7), p.1238-1242
Main Authors: Leonello, Rocco, Savio, Matteo, Baron Toaldo, Paola, Bonomi, Renato
Format: Article
Language:English
Subjects:
Analytical chemistry
Biochemistry
Chemistry
College Science
College students
Colleges & universities
Enzymatic activity
Enzymes
Foreign Countries
Galactose
High Schools
Kinetics
Laboratory Experiments
Lactase
Lactose
Measuring instruments
Organic chemistry
Parameters
Reagents
Science Experiments
Science Instruction
Science Laboratories
Scientific Concepts
Secondary School Science
Sugar
Titration
Undergraduate Study
Volumetric analysis
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Summary:Determination of enzymatic activity is a relevant experiment in teaching chemistry and biochemistry to high school and college students. A method for measuring the kinetic parameters of lactose cleavage by the enzyme lactase is described. This enzyme catalyzes the hydrolysis of lactose to galactose and glucose. The kinetic parameters can be determined easily and quickly by titration using Fehling’s test for reducing sugars. In this way, it is possible to determine the rate of the reaction without using any instruments.
ISSN:0021-9584
1938-1328
DOI:10.1021/acs.jchemed.7b00637