Protein Colorimetry Experiments That Incorporate Intentional Discrepancies and Historical Narratives

A set of biochemistry experiments based on the technique of protein colorimetry is described. Students used the Biuret and Bradford assays to qualitatively and quantitatively assess solutions for protein. Two unique features of these experiments are (1) the use of intentional discrepancies within ea...

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Bibliographic Details
Published in:Journal of chemical education 2018-07, Vol.95 (7), p.1198-1204
Main Authors: Astrof, Nathan S, Horowitz, Gail
Format: Article
Language:English
Subjects:
Biochemistry
Colorimetry
Constructivism
Continuing education
Critical Thinking
Laboratory Training
Narratives
Proteins
Science Experiments
Science Instruction
Science Laboratories
Scientific Concepts
Teaching Methods
Undergraduate Study
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Summary:A set of biochemistry experiments based on the technique of protein colorimetry is described. Students used the Biuret and Bradford assays to qualitatively and quantitatively assess solutions for protein. Two unique features of these experiments are (1) the use of intentional discrepancies within each experiment to promote critical thinking and (2) the use of authentic historical narratives to introduce critical concepts in the interpretation of assays and demonstrate the relevance of laboratory work to “real-world” scenarios. Details on the implementation and the evaluation of these experiments are discussed.
ISSN:0021-9584
1938-1328
DOI:10.1021/acs.jchemed.7b00633