Size Exclusion Chromatography: A Teaching Aid for Physical Chemistry

All analytical techniques are governed by the principles of physical chemistry, and chromatography is certainly no exception. By examining the underlying concepts of size exclusion chromatography (SEC), students can gain greater appreciation of and insight into the science and utility of thermodynam...

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Bibliographic Details
Published in:Journal of chemical education 2018-07, Vol.95 (7), p.1125-1131
Main Author: Barth, Howard G
Format: Article
Language:English
Subjects:
Analytical chemistry
Chemistry
Chromatography
College Science
Curricula
High performance liquid chromatography
Inquiry
Instructional aids
Liquid chromatography
Macromolecules
Organic chemistry
Physical chemistry
Science Instruction
Scientific Concepts
Size exclusion chromatography
Thermodynamics
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Summary:All analytical techniques are governed by the principles of physical chemistry, and chromatography is certainly no exception. By examining the underlying concepts of size exclusion chromatography (SEC), students can gain greater appreciation of and insight into the science and utility of thermodynamics, as well as solution properties of macromolecules. In this paper we will show how the separation mechanism that governs SEC, an unusual form of chromatography, can be explained using the tenets of thermodynamics. This paper gives educators access to an HPLC technique usually not included in undergraduate chemistry curriculum.
ISSN:0021-9584
1938-1328
DOI:10.1021/acs.jchemed.8b00171