Gold nanoparticle-decorated reduced-graphene oxide targeting anti hepatitis B virus core antigen

• Published in: Bioelectrochemistry (Amsterdam, Netherlands) Netherlands), 2018-08, Vol.122, p.199-205
• Main Authors: Abd Muain, Mohamad Farid, Cheo, Kooi Hoong, Omar, Muhamad Nadzmi, Amir Hamzah, Amir Syahir, Lim, Hong Ngee, Salleh, Abu Bakar, Tan, Wen Siang, Ahmad Tajudin, Asilah
• Format: Article
• Language: English
• Subjects: 17β-Estradiol; Antibody; Antigens; Biosensing Techniques - methods; Bovine serum albumin; Core protein; Electrochemistry; Electron transfer; Gold; Gold - chemistry; Gold nanoparticles; Graphene; Graphite - chemistry; Hepatitis; Hepatitis B; Hepatitis B - blood; Hepatitis B - diagnosis; Hepatitis B - virology; Hepatitis B Core Antigens - analysis; Hepatitis B Core Antigens - blood; Hepatitis B virus; Hepatitis B virus - isolation & purification; Humans; Immobilization; Immunoglobulins; Impedimetric biosensor; Limit of Detection; Metal Nanoparticles - chemistry; Nanocomposites; Nanoparticles; Oxidation-Reduction; Oxides - chemistry; Proteins; Reduced graphene oxide; Serum albumin; Sex hormones; Viruses
• ISSN: 1567-5394; 1878-562X
• DOI: 10.1016/j.bioelechem.2018.04.004

Hepatitis B virus core antigen (HBcAg) is the major structural protein of hepatitis B virus (HBV). The presence of anti-HBcAg antibody in a blood serum indicates that a person has been exposed to HBV. This study demonstrated that the immobilization of HBcAg onto the gold nanoparticles-decorated reduced graphene oxide (rGO-en-AuNPs) nanocomposite could be used as an antigen-functionalized surface to sense the presence of anti-HBcAg. The modified rGO-en-AuNPs/HBcAg was then allowed to undergo impedimetric detection of anti-HBcAg with anti-estradiol antibody and bovine serum albumin as the interferences. Upon successful detection of anti-HBcAg in spiked buffer samples, impedimetric detection of the antibody was then further carried out in spiked human serum samples. The electrochemical response showed a linear relationship between electron transfer resistance and the concentration of anti-HBcAg ranging from 3.91ngmL−1 to 125.00ngmL−1 with lowest limit of detection (LOD) of 3.80ngmL−1 at 3σm−1. This established method exhibits potential as a fast and convenient way to detect anti-HBcAg. [Display omitted] •Protein-functionalized graphene-based nanocomposite to sense anti-HBcAg•Limit of detection of 3.80ngmL−1 in spiked samples•Protein-functionalized electrode is selective and specific towards anti-HBcAg.•Works on diluted human serum sample