Carbon nanotube-based electrochemical biosensors for determination of Candida albicans’s quorum sensing molecule

[Display omitted] •Electrochemical biosensors developments for pathogenic detections.•Synthesis, characterizations and electrocatalytic activity investigation of Crown-ether/MWCNTs modified sensors.•Assay optimization, validation and determination of a quorum sensing molecule (Tryptophol) in Candida...

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Published in:Sensors and actuators. B, Chemical Chemical, 2017-06, Vol.244, p.565-570
Main Authors: Hassan, Rabeay Y.A., El-Attar, Rehab O., Hassan, Hassan N.A., Ahmed, Mona A., Khaled, Elmorsy
Format: Article
Language:English
Subjects:
Biofilms
Biological activity
Biosensors
Buffer solutions
Candida albicans
Carbon nanotubes
Chemical sensors
Crown ethers
Detection
Electrochemical biosensors
Electrolytes
Multi wall carbon nanotubes
Nanomaterials
Nanotubes
Quorum sensing molecule
Regression analysis
Regression coefficients
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Summary:[Display omitted] •Electrochemical biosensors developments for pathogenic detections.•Synthesis, characterizations and electrocatalytic activity investigation of Crown-ether/MWCNTs modified sensors.•Assay optimization, validation and determination of a quorum sensing molecule (Tryptophol) in Candida albicans’s culture. Candida albicans produces various quorum sensing molecules (QSMs) which are involved in biological processes, e.g. morphological changes or biofilm formation. Thus, a sensitive and selective electrochemical assay was developed for determination of tryptophol as one of the most important QSMs. Preparation, characterization, and testing the electrocatalytic activity of prepared sensors with various sensing elements and nanomaterials were investigated. In addition, the effects of supporting electrolytes, pH of buffer solutions, scan rate, and possible interferences on the response of tryptophol’s sensor were studied. Eventually, improving the sensitivity and selectivity were achieved using a combination of 12-crwon-4-ether and MWCNTs (10% w/w for each). With the developed electrochemical sensor, the linear response was observed from 10μg/ml to 110μg/ml with the detection limit of 1μg/ml and regression coefficient of 0.998. Consequently, the method was successfully applied for the detection of tryptophol in the fungal culture.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2017.01.028