Loading…
Identification and Characterization of ATP/ADP Isopentenyltransferases (ATP/ADP PpIPTs) Genes in Peach
ATP / ADP isopentenyltransferase ( IPTs ) genes encode key enzymes involved in cytokinin synthesis. In this study, the functions of ATP / ADP PpIPTs in peach were investigated. According to the genome sequence, we have found and verified that there are four members of this gene family in peach, name...
Saved in:
Published in: | Journal of plant growth regulation 2019-06, Vol.38 (2), p.416-430 |
---|---|
Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | ATP
/
ADP isopentenyltransferase
(
IPTs
) genes encode key enzymes involved in cytokinin synthesis. In this study, the functions of
ATP
/
ADP PpIPTs
in peach were investigated. According to the genome sequence, we have found and verified that there are four members of this gene family in peach, namely,
PpIPT1, PpIPT3, PpIPT5
, and
PpIPT7
. Overexpression of each of these genes in
Arabidopsis
resulted in increased levels of cytokinins in the transgenic plants, confirming their roles in cytokinin synthesis. Numerous altered phenotypes were observed in the transgenic plants, including vigorous growth and enhanced salt resistance.
ATP
/
ADP PpIPTs
were expressed in tissues throughout the plant, but the expression patterns differed between the genes. Only
PpIPT3
was upregulated within 2 h after the application of nitrate to N-deprived peach seedlings, and the increase was resistant to pre-treatment of a specific nitrate metabolism inhibitor. Results showed that
ATP
/
ADP PpIPT
expression levels decreased significantly in pulp within 2 weeks after flowering and remained low. However, pulp cytokinin levels were quite high during this time. Only
PpIPT5
in seed increased significantly within 2 weeks after flowering, which was consistent with cytokinin levels during early fruit development, suggesting that
PpIPT5
in seed is the key gene for cytokinin biosynthesis during early fruit development.
ATP
/
ADP PpIPT
expression also increased significantly during later fruit development in seed. |
---|---|
ISSN: | 0721-7595 1435-8107 |
DOI: | 10.1007/s00344-018-9851-6 |