Effects of Wheat and Faba Bean Intercropping on Microorganism Involved in Nitrogen Transformation in the Rhizosphere Soils

• Published in: Journal of agricultural resources and environment 2016-01, Vol.33 (5), p.482
• Main Authors: Yan-Fen, Tang, Yong-Bo, Xu, Zheng, Yi, Bao-Kun, Lei
• Format: Article
• Language: Chinese
• Subjects: Abundance; Actinobacteria; Actinomyces; Aerobic bacteria; Agricultural land; Agricultural practices; Agriculture; Ammonium; Anaerobic bacteria; Anaerobic microorganisms; Bacteria; Biochemistry; Biology; Community structure; Corn; Crop diseases; Crops; Elongation; Environmental changes; Fatty acids; Fertilizers; Fungi; Genes; Intercropping; Measurement methods; Microorganisms; Monoculture; Nitrates; Nitrogen; Nitrogen cycle; Nitrous oxide; NorB protein; Nutrition; Phospholipids; Polymerase chain reaction; Rhizosphere; Rhizosphere microorganisms; Soil conservation; Soil fertility; Soil microorganisms; Soil pollution; Soil sciences; Soil structure; Wheat
• ISSN: 2095-6819; 2095-6819
• DOI: 10.13254/j.jare.2016.0083

Soil microorganism is one of the key factors that affects soil ecological activity. It is an important symbol of soil health, and the soil nitrogen cycle is closely related to the microorganisms. The relationship between nitrogen and microorganisms under the intercropping is im-portant for the farmland ecosystem. In this paper, phospholipid fatty acids(PLFA) analysis was used to determine soil microbial communi-ties, e.g., biomasses of anaerobic bacteria, aerobic bacteria, bacteria, fungi and actinobacteria. The abundance of nitrifying genes (AOB, AOA) and three denitrifying genes (nirK, norB, nosZ) were measured using real-time quantitative polymerase chain reaction. The enzymes, nitrate and ammonium concentrations were measured using conventional methods. The results showed that along with the growth period, the TPLFAs (total phospholipid fatty acids) increased and the bacterias, fungus, actinomyces and aerobic bacterias significantly (P