A nanostructured electrochemical aptasensor for highly sensitive detection of chlorpyrifos

A sensitive and selective electrochemical aptasensor based on a novel composite film consisting of carbon black (CB) and graphene oxide@Fe3O4was developed for detection of chlorpyrifos. Chitosan (CS) functionalized CB has high specific surface area, ideal dispersibility and good electrical conductiv...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2017-05, Vol.243, p.1164-1170
Main Authors: Jiao, Yancui, Hou, Wenjie, Fu, Jiayun, Guo, Yemin, Sun, Xia, Wang, Xiangyou, Zhao, Jing
Format: Article
Language:English
Subjects:
Aptasenor
Biomolecules
Carbon black
Chitosan
Chlorpyrifos
Conductivity
Electrical resistivity
Electron transfer
Fe3O4
Graphene
Graphene oxide
Iron oxides
Molecules
Nanocomposites
Nanostructured materials
Peptides
Pesticides
Reproducibility
Selectivity
Sensors
Surface area
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Summary:A sensitive and selective electrochemical aptasensor based on a novel composite film consisting of carbon black (CB) and graphene oxide@Fe3O4was developed for detection of chlorpyrifos. Chitosan (CS) functionalized CB has high specific surface area, ideal dispersibility and good electrical conductivity, thus, we used them to capture more graphene oxide@Fe3O4 and further amplify the current signal of the aptasensor. The graphene oxide@Fe3O4 nanocomposite formed a unique sensing film with strong synergistic effects, the graphene oxide provided a large surface area, whereas Fe3O4 was uniformly deposited on the graphene oxide, facilitating electron transfer for sensitive detection of chlorpyrifos with excellent selectivity and a low detection limit. Immobilization of the probe Apt on the electrode was greatly improved owing to the unique synergistic effects of graphene oxide@Fe3O4. Therefore, the integration of graphene oxide@Fe3O4 nanocomposite with biomolecules chlorpyrifos aptamer (Apt) as a biorecognition element constructed a novel biosensing platform. Under the optimized conditions, the proposed aptasensor provided a linear range of 0.1–105ng/mL with a low detection limit of 0.033ng/mL. The aptasensor performed good selectivity, reproducibility and stability. Additionally, the proposed aptasensor can be applied to monitor chlorpyrifos residues in real vegetable samples.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2016.12.106