A half-type ABC transporter FeSTAR1 regulates Al resistance possibly via UDP-glucose-based hemicellulose metabolism and Al binding

Aims Buckwheat (Fagopyrum esculentum) is highly tolerant to Al stress, but the molecular mechanisms remain largely unknown. This study aims to investigate a half-type ABC transporter gene (FeSTAR1) with respect to Al tolerance. Methods The expression of FeSTAR1 was examined and complementation test...

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Bibliographic Details
Published in:Plant and soil 2018-11, Vol.432 (1/2), p.303-314
Main Authors: Xu, Jia Meng, Lou, He Qiang, Jin, Jian Feng, Chen, Wei Wei, Wan, Jiang Xue, Fan, Wei, Yang, Jian Li
Format: Article
Language:English
Subjects:
ABC transporter
ABC transporters
Aluminum
Aluminum (Metal)
Binding
Biomedical and Life Sciences
Buckwheat
Cell walls
Complementation
Cytoplasm
Ecology
Gene expression
Glucose
Glucose metabolism
Health aspects
Hemicellulose
Life Sciences
Metabolism
Molecular modelling
Plant metabolism
Plant Physiology
Plant Sciences
Polysaccharides
Protein transport
Proteins
Regular Article
Saccharides
Soil Science & Conservation
Transport proteins
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Summary:Aims Buckwheat (Fagopyrum esculentum) is highly tolerant to Al stress, but the molecular mechanisms remain largely unknown. This study aims to investigate a half-type ABC transporter gene (FeSTAR1) with respect to Al tolerance. Methods The expression of FeSTAR1 was examined and complementation test in atstar1 mutant was conducted. Furthermore, Al distribution and cell wall polysaccharides content were analyzed. Results FeSTAR1 is an ABC transporter protein with nucleotide binding domain, but lack of transmembrane domain. Consistently, FeSTAR1 is a soluble protein, localizing to both cytoplasm and nucleus. Al rapidly and specifically induced FeSTAR1 expression. Heterologous expression of FeSTAR1 in atstar1 rescued its Al tolerance, and exogenous applied UDP-glucose could alleviate Al sensitivity of atstar1 mutant, suggesting the connection between FeSTAR1 and UDP-glucose in terms of Al tolerance. Furthermore, FeSTAR1 complemented lines accumulated less Al in root cell wall than atstar1 mutant. Further cell wall fraction analysis showed that Al was largely confined to cell wall hemicellulose1, at which Al content was significantly lower in complemented lines. Consistent with Al distribution in different cell wall polysaccharides, complemented lines had lower hemicellulosel content. Conclusion Our results indicate that FeSTAR1 is involved in Al resistance via possibly cell wall matrix polysaccharides metabolism in buckwheat.
ISSN:0032-079X
1573-5036
DOI:10.1007/s11104-018-3805-4