A Green BODIPY‐Based, Super‐Fluorogenic, Protein‐Specific Labelling Agent

We report the development of YC23, a novel green BODIPY‐based dimaleimide derivative that undergoes a fluorogenic addition reaction (FlARe) with a genetically encodable peptide tag (dC10α) that can be fused to a protein of interest (POI). We also demonstrate the application of this reaction for the...

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Bibliographic Details
Published in:Angewandte Chemie 2018-09, Vol.130 (38), p.12570-12574
Main Authors: Chen, Yingche, Tsao, Kelvin, Acton, Sydney L., Keillor, Jeffrey W.
Format: Article
Language:English
Subjects:
BODIPY
Chemistry
Fluorogene Sonden
Labeling
Maleimid
Mammalian cells
Proteinmarkierung
Proteins
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Summary:We report the development of YC23, a novel green BODIPY‐based dimaleimide derivative that undergoes a fluorogenic addition reaction (FlARe) with a genetically encodable peptide tag (dC10α) that can be fused to a protein of interest (POI). We also demonstrate the application of this reaction for the fluorogenic labelling of a specific POI in bacterial lysate and in living mammalian cells. Fluoreszenzverstärkung: Eine Dimaleimid‐BODIPY‐Sonde wurde entwickelt, die die hochfluorogene Markierung eines spezifischen Peptid‐Tags, der gentechnisch an ein Protein fusioniert werden kann, in lebenden Säugerzellen ermöglicht.
ISSN:0044-8249
1521-3757
DOI:10.1002/ange.201805482