Wrinkle-free tissue sections

The aim was to compare the efficiency of routine method, isopropyl alcohol, and acetic acid solutions for removing wrinkles/folds from the tissue sections obtained. Formalin-fixed paraffin-embedded tissue blocks of six oral tissues, two uterus (cervix) tissues, and two skin tissues were employed. Di...

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Bibliographic Details
Published in:Journal of Advanced Clinical and Research Insights 2015-03, Vol.2 (2), p.78-81
Main Authors: Raju, Shashidara, Sridhara, Sudheendra Udyavara, Gopalkrishna, Aparna H., Haragannavar, Vanishri C., Mirshad, Reshma, Latha, D.
Format: Article
Language:English
Subjects:
Acids
Adhesives
Alcohol
Biopsy
Laboratories
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Summary:The aim was to compare the efficiency of routine method, isopropyl alcohol, and acetic acid solutions for removing wrinkles/folds from the tissue sections obtained. Formalin-fixed paraffin-embedded tissue blocks of six oral tissues, two uterus (cervix) tissues, and two skin tissues were employed. Dilute isopropyl alcohol solution was prepared by mixing one volume of isopropyl alcohol with 15 volumes of distilled water, 30 mM and 40 mM of acetic acid were also used and were employed as flotation media. In our study, sections lifted from the water baths, after floatation onto all three different media, yielded largely similar results. In the case of oral tissues, alcohol seemed to give marginally better results. Dilute alcohol and acetic acid are the alternate floatation media used in this study, but the control group gave comparatively better results.
ISSN:2393-8625
2393-8625
DOI:10.15713/ins.jcri.49