Expression of the B-Cell Lymphoma/Leukemia 11A Gene in Malignant Hematological Cell Lines through Quantitative Reverse Transcription Polymerase Chain Reaction

OBJECTIVE The B-cell lymphoma/leukemia 11A (BCL11A) gene is essential for normal lymphoid development and has been associated with hematological malignancies. In the current study, the relative expression level of BCL11A in malignant hematological cell lines was evaluated through real-time quantitat...

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Bibliographic Details
Published in:Cancer biology & medicine 2011-12, Vol.8 (4), p.242
Main Authors: Yang-jun, Gao, Dong-mei, He, Shao-hua, Chen, Xiao-juan, Yan, Xiao-mao, Hu, Yang-qiu, Li
Format: Article
Language:English
Subjects:
Acute myeloid leukemia
B-cell lymphoma
Gene expression
Glyceraldehyde
Hematology
Leukemia
Lymphocytes B
Lymphoma
Myeloid leukemia
Polymerase chain reaction
Reverse transcription
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Summary:OBJECTIVE The B-cell lymphoma/leukemia 11A (BCL11A) gene is essential for normal lymphoid development and has been associated with hematological malignancies. In the current study, the relative expression level of BCL11A in malignant hematological cell lines was evaluated through real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR). METHODS The relative expression level of BCL11A mRNA in malignant hematological cell lines was determined through qRT-PCR using SYBR Green I dye. Glyceraldehyde-3-phosphate dehydro-genase was used as the reference gene to confirm the relative expression level of BCL11A gene mRNA. RESULTS The relative expression level of BCL11A mRNA in cell lines from B-cell malignancies was significantly higher compared with that from acute myeloid leukemia (P
ISSN:2095-3941