Effects of 125I-Labeled Peptide Nuclear Acid Targeting Ki67 on the Growth of Implanted Human Renal Cell Carcinoma in Nude Mice

OBJECTIVE To investigate the potential of 125I-labeled anti-sense peptide nucleic acids (125I-AS-PNAs) to inhibit the expression of the Ki-67 gene and growth of implanted human renal carcinoma cells in nude mice. METHODS Anti-sense peptide nucleic acids (AS-PNAs) targeting the Ki67 gene were synthes...

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Bibliographic Details
Published in:Cancer biology & medicine 2004-12, Vol.1 (6), p.448
Main Authors: Chen, Jiacun, Zheng, Junnian, Wu, Song, Lai, Haibiao, Sun, Xiaoqing, Liu, Junjie
Format: Article
Language:English
Subjects:
Antisense DNA
Apoptosis
Ki-67 gene
Peptide nucleic acids
Peptides
Radiation therapy
Renal cell carcinoma
Tumor cells
Tumor suppression
Tumors
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Summary:OBJECTIVE To investigate the potential of 125I-labeled anti-sense peptide nucleic acids (125I-AS-PNAs) to inhibit the expression of the Ki-67 gene and growth of implanted human renal carcinoma cells in nude mice. METHODS Anti-sense peptide nucleic acids (AS-PNAs) targeting the Ki67 gene were synthesized and labeled with 125I by the Chloraseptine-T method. Drugs including PNAs and 125I-AS-PNAs capsulated by cationic lipid were directly injected into tumors in nude mice. The Ki67 expression in tumors was detected by an immunohistochemical technique and Western blot. The apoptosis of tumor cells was detected by a TUNEL assay. Tumor volumes were measured every 3 days and tumor suppression rates were calculated at 12 days after treatment. Control groups were treated with AS-PNA, MMPNAs (mismatch PNAs) and 125I-Na. RESULTS The Ki67 expression rate of tumors treated by 125I-AS-PNAs [(15.3±1.8)%] was lower than that treated by AS-PNAs [(23.0±2.4)%] (P
ISSN:2095-3941