Preparation of Chitosan Cryostructurates with Controlled Porous Morphology and Their Use as 3D-Scaffolds for the Cultivation of Animal Cells

The influence of the conditions of the formation of genipin cross-linked chitosan cryostructurates on the porous morphology and physicochemical properties of these cryostructurates and on the possibility of their use as biopolymer 3D scaffolds for tissue engineering was studied. The chitosan cryostr...

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Bibliographic Details
Published in:Applied biochemistry and microbiology 2018-09, Vol.54 (5), p.459-467
Main Authors: Sazhnev, N. A., Drozdova, M. G., Rodionov, I. A., Kil’deeva, N. R., Balabanova, T. V., Markvicheva, E. A., Lozinsky, V. I.
Format: Article
Language:English
Subjects:
Acetic acid
Amino groups
Ammonia
Biochemistry
Biocompatibility
Biodegradation
Biomedical and Life Sciences
Biopolymers
Chitosan
Crosslinking
Cultivation
Evaporation
Fibroblasts
Freeze drying
Genipin
Hydrogels
Laser microscopy
Life Sciences
Medical Microbiology
Microbiology
Morphology
Physicochemical properties
Polymers
Scaffolds
Tissue engineering
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Summary:The influence of the conditions of the formation of genipin cross-linked chitosan cryostructurates on the porous morphology and physicochemical properties of these cryostructurates and on the possibility of their use as biopolymer 3D scaffolds for tissue engineering was studied. The chitosan cryostructurates were obtained by freeze-drying a chitosan acetate solution, treating the resulting sponge with an alcohol solution of ammonia to transform the polyaminosaccharide from a salt into a chitosan-base, and then cross-linking the polymer with genipin (the molar ratios of genipin to the number of chitosan amino groups were 0.05, 0.033, and 0.02, respectively). The pore sizes, water-holding capacity, and in vitro biodegradation rate of the cryostructurates were shown to depend on the aforementioned ratio. The properties of the prepared chitosan cryostructurates, the hydrogels formed by chitosan cross-linking with genipin at positive temperatures, and the films cast from genipin-containing chitosan solutions after solvent evaporation were studied and compared. The biocompatibility of the obtained macroporous sponge materials was demonstrated using L929 mouse fibroblasts. Confocal laser microscopy showed that the cells in all of the 3D scaffolds obtained were evenly distributed; they grew and proliferated when cultured in vitro for seven days.
ISSN:0003-6838
1608-3024
DOI:10.1134/S0003683818050162