Apolipoprotein D in the aging brain and in Alzheimer's dementia

Apolipoprotein D (apoD) levels were examined in the temporal cortex as well as an assessment of the location of apoD positive cells within the brain by immunohistochemical and biochemical methods in young control (YC), aged control (AC), and Alzheimer's demented (AD) probands. Scattered apoD po...

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Bibliographic Details
Published in:Neurological research (New York) 2000-06, Vol.22 (4), p.330-336
Main Authors: Kalman, K., McConathy, W., Araoz, C., Kasa, P., Lacko, Andras G.
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Aging
Aging - pathology
Alzheimer Disease - pathology
Alzheimer's dementia
Apolipoprotein
Apolipoproteins - analysis
Apolipoproteins D
Astrocytes - chemistry
Astrocytes - pathology
Blotting, Western
Brain
Brain - pathology
Brain Chemistry
Dementia
Humans
Immunohistochemistry
Middle Aged
Oligodendroglia - chemistry
Oligodendroglia - pathology
Pyramidal Cells - chemistry
Pyramidal Cells - pathology
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Summary:Apolipoprotein D (apoD) levels were examined in the temporal cortex as well as an assessment of the location of apoD positive cells within the brain by immunohistochemical and biochemical methods in young control (YC), aged control (AC), and Alzheimer's demented (AD) probands. Scattered apoD positive astrocytes and oligodendrocytes were found throughout the white matter by immunohistochemistry. ApoD immunoreactivity was also observed In the cerebellar oligodendrocytes of the YC group. There was faint positive apoD staining in scattered cortical astrocytes and a few neurons in the same group. In contrast, some of the AC and all of the AD probands had intense and frequent apoD immunostained cortical astrocytes and pyramidal neurons. The cortical senile plaques and neurofibrillary tangles were apoD immunonegative. No quantitative differences were found between the cortical apoD levels in the AC and AD groups, determined by immunoblotting. ApoD detected in the brain tissue was different in molecular weight (29 kDal) from that seen in CSF or in the serum (32 kDal). Our results indicate apoD is present in the human brain, especially in glial cells, and has increased abundance in the elderly and AD subjects [Neurol Res 2000; 22: 330-336]
ISSN:0161-6412
1743-1328
DOI:10.1080/01616412.2000.11740678