Human surfactant proteins A1 and A2 are differentially regulated during development and by soluble factors

An RT-PCR method for the relative quantitation of the mRNAs RT-PCR method for human surfactant protein (SP) Al and SP-A2 was developed, verified, and then utilized to determine the relative levels of these mRNAs in fetal and adult lung samples in vivo, as well as in cultured human fetal lung explant...

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Bibliographic Details
Published in:American journal of physiology. Lung cellular and molecular physiology 1998-10, Vol.19 (4), p.L653-L669
Main Authors: SCAVO, L. M, ERTSEY, R, BI QI GAO
Format: Article
Language:English
Subjects:
Biological and medical sciences
Embryology: invertebrates and vertebrates. Teratology
Fundamental and applied biological sciences. Psychology
Lungs
Organogenesis. Fetal development
Organogenesis. Physiological fonctions
Prenatal development
Proteins
Ribonucleic acid
RNA
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Summary:An RT-PCR method for the relative quantitation of the mRNAs RT-PCR method for human surfactant protein (SP) Al and SP-A2 was developed, verified, and then utilized to determine the relative levels of these mRNAs in fetal and adult lung samples in vivo, as well as in cultured human fetal lung explants and H441 cells. For the cultured tissue and cells, we assessed the effects of a variety of soluble factors known to modulate total SP-A.
ISSN:1040-0605
1522-1504