Establishment of an immortalized fetal intrapulmonary artery endothelial cell line

In an effort to establish an immortalized cell line, ovine fetal pulmonary artery endothelial cells (PAECs; passage 5) were permanently transfected with the E6 and E7 open reading frames of human papillomavirus type 16, and phenotypes related to nitric oxide (NO) production were evaluated up to pass...

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Bibliographic Details
Published in:American journal of physiology. Lung cellular and molecular physiology 1999-07, Vol.21 (1), p.L106-L112
Main Authors: PACE, M. C, CHAMBLISS, K. L, GERMAN, Z, YUHANNA, I. S, MENDELSOHN, M. E, SHAUL, P. W
Format: Article
Language:English
Subjects:
Animal cells
Biological and medical sciences
Biotechnology
Blood vessels
Cells
Establishment of new cell lines, improvement of cultural methods, mass cultures
Eukaryotic cell cultures
Fetuses
Fundamental and applied biological sciences. Psychology
Genes
Lungs
Methods. Procedures. Technologies
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Summary:In an effort to establish an immortalized cell line, ovine fetal pulmonary artery endothelial cells (PAECs; passage 5) were permanently transfected with the E6 and E7 open reading frames of human papillomavirus type 16, and phenotypes related to nitric oxide (NO) production were evaluated up to passage 28.
ISSN:1040-0605
1522-1504