In vivo and in vitro uptake of surfactant lipids by alveolar type II cells and macrophages

The uptake of fluorescent-labeled liposomes (with a surfactant-like composition) by alveolar macrophages and alveolar type II cells was studied using flow cytometry, in vivo by instillation of the labeled liposomes in the trachea of ventilated rats followed by isolation of the alveolar cells and det...

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Bibliographic Details
Published in:American journal of physiology. Lung cellular and molecular physiology 2002-09, Vol.27 (3), p.L648-L654
Main Authors: POELMA, D. L. H, ZIMMERMANN, L. J. I, SCHOLTEN, H. H, LACHMANN, B, VAN IWAARDEN, J. F
Format: Article
Language:English
Subjects:
Air breathing
Biological and medical sciences
Cells
Cellular biology
Fundamental and applied biological sciences. Psychology
Respiratory system
Respiratory system: anatomy, metabolism, gas exchange, ventilatory mechanics, respiratory hemodynamics
Rodents
Vertebrates: respiratory system
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Summary:The uptake of fluorescent-labeled liposomes (with a surfactant-like composition) by alveolar macrophages and alveolar type II cells was studied using flow cytometry, in vivo by instillation of the labeled liposomes in the trachea of ventilated rats followed by isolation of the alveolar cells and determination of the cell-associated fluorescence, and in vitro by incubation of isolated alveolar cells with the fluorescent liposomes. The results show that the uptake of liposomes by the alveolar cells is time and concentration dependent.
ISSN:1040-0605
1522-1504