Loading…

Improved photodynamic effect through encapsulation of two photosensitizers in lipid nanocapsules

Photodynamic therapy (PDT) has developed into a new clinical and non-invasive treatment for cancer over the past 30 years. By the combination of three non-toxic partners, i.e. a photosensitizer (PS), molecular oxygen (O 2 ) and light, cytotoxic reactive oxygen species (ROS) are locally produced lead...

Full description

Saved in:
Bibliographic Details
Published in:Journal of materials chemistry. B, Materials for biology and medicine Materials for biology and medicine, 2018, Vol.6 (37), p.5949-5963
Main Authors: Barras, Alexandre, Skandrani, Nadia, Gonzalez Pisfil, Mariano, Paryzhak, Solomiya, Dumych, Tetiana, Haustrate, Aurélien, Héliot, Laurent, Gharbi, Tijani, Boulahdour, Hatem, Lehen'kyi, V'yacheslav, Bilyy, Rostyslav, Szunerits, Sabine, Bidaux, Gabriel, Boukherroub, Rabah
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Photodynamic therapy (PDT) has developed into a new clinical and non-invasive treatment for cancer over the past 30 years. By the combination of three non-toxic partners, i.e. a photosensitizer (PS), molecular oxygen (O 2 ) and light, cytotoxic reactive oxygen species (ROS) are locally produced leading to irreversible vascular and cellular damage. In the present study, we report for the first time that the combination of two photosensitizers (2 PSs: Protoporphyrin IX, PpIX and Hypericin, Hy) loaded in the same lipid nanocapsules (LNCs) leads to enhanced photodynamic therapy efficiency when compared with previously reported systems. The 2 PS-loaded LNCs are shown to increase the in vitro phototoxicity at the nanomolar range (IC 50 = 274 and 278 nM on HeLa and MDA-MB-231 cell lines, respectively), whereas the corresponding single PS-loaded LNCs at the same concentration exhibit a phototoxicity two times lower. Intracellular localization in HeLa cells indicates a subcellular asymmetry of PpIX and Hy, in the plasma, ER membranes and round internal structures. The biodistribution of LNCs was studied upon different routes of injection into Swiss nude mice; based on the obtained data, LNCs were injected intratumorally and used to slow the growth of xenograft tumors in mice. The results obtained in this study suggest that the combination of two or more PSs may be a promising strategy to improve the efficacy of conventional photodynamic therapy as well as to reduce dark toxicity. Encapsulation of two photosensitizers in lipid nanocapsules leads to enhanced photodynamic therapy efficiency.
ISSN:2050-750X
2050-7518
DOI:10.1039/c8tb01759j