Comparison of Gastric Inflammation in Balb/c Inoculated by Three Unique EPIYT Motif CagA Gene Helicobacter pylori Bali Isolates Based on Update Sidney System Criteria

Helicobacter pylori infection ( H. pylori ) is associated with gastric carcinoma and various of gastrointestinal disease. The prevalence of H. pylori infection in Indonesia is still controversial. There is no data about H. pylori Bali isolates and the Cag A virulence factor from patients with dyspep...

Full description

Saved in:
Bibliographic Details
Published in:Biomedical & pharmacology journal 2017, Vol.10 (3), p.1401-1408
Main Authors: Wirawan, I. Made Suma, Muttaqin, Zainul, Novitasari, Novitasari, Widiana, I. Gde Raka, Artha, I. Gst Alit, Mantik, I. Nyoman
Format: Article
Language:English
Subjects:
Amino acids
Biomedical research
Biopsy
CagA protein
Deoxyribonucleic acid
DNA
Dyspepsia
Gastric cancer
Gastroenterology
Gel electrophoresis
Helicobacter pylori
Infections
Inflammation
Lymphoma
Metabolism
Pathogenesis
Phylogenetics
Studies
Trinucleotide repeats
Ulcers
Virulence factors
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Helicobacter pylori infection ( H. pylori ) is associated with gastric carcinoma and various of gastrointestinal disease. The prevalence of H. pylori infection in Indonesia is still controversial. There is no data about H. pylori Bali isolates and the Cag A virulence factor from patients with dyspepsia in Bali. H. pylori Bali isolates provided from Laboratorium of Microbiology, Biomedical Research Unit, West Nusatenggara General Hospital, which have been isolated from gastric antral biopsies collected from 3 gastric ulcer Balinese patients. This research revealed that all of three H. pylori Bali isolates examined positive for CagA (Cytotoxin associated antigen A)gene. Based on the result of the gel electrophoresis, a 640 bp single band observed similar in each sample. All H. pylori Bali isolates have EPIYA-ABD motif with unique motifs EPIYT on second EPIYA sequence.Three of H. pylori Bali isolates named as BLI 01, BLI 02 and BLI 03 that have a CagA genes. All the H. pylori Bali isolates have an unique EPIYT motifs (substitution A to T) in CagA genes. The degree of gastric inflammation is more severe in the 4 th week of the study. There was a significant difference of gastric inflammation degree in all of three groups of mice. H. pylori BLI 03 isolate with positive CagA gene, have a greatest degree of inflammation.
ISSN:0974-6242
2456-2610
DOI:10.13005/bpj/1245