In-vitro anti-cancer activity of organic template-free hierarchical M (Cu, Ni)-modified ZSM-5 zeolites synthesized using silica source waste material

• Published in: Journal of photochemistry and photobiology. B, Biology Biology, 2018-09, Vol.186, p.178-188
• Main Authors: Jesudoss, S.K., Judith Vijaya, J., Kaviyarasu, K., Iyyappa Rajan, P., Narayanan, S., John Kennedy, L.
• Format: Article
• Language: English
• Subjects: Anti-cancer; Anticancer properties; Apoptosis; Biocompatibility; Biomedical materials; Biotechnology; Cancer; Cell death; Chemical synthesis; Copper; Cytotoxicity; Deoxyribonucleic acid; DNA; DNA damage; Fourier transforms; High resolution; Hydrothermal; Hydrothermal treatment; Infrared spectroscopy; Lung cancer; Metal modified ZSM-5; Metals; MTT assay; Nickel; Oxidative stress; Physical properties; Rice husk ash; Scanning electron microscopy; Silica; Silicon dioxide; Thermogravimetric analysis; Toxicity; Transmission electron microscopy; Tumor cell lines; Waste materials; X-ray diffraction; Zeolites
• ISSN: 1011-1344; 1873-2682
• DOI: 10.1016/j.jphotobiol.2018.07.009

The present paper is focused on a simple and economical route to synthesize the organic template-free hierarchical pure and M (Cu, Ni)-modified ZSM-5 zeolites (1%, 3% and 5%) using hydrothermal treatment in the presence of silica rich rice husk ash and its application studies through the evaluation of their anti-cancer activity on A459 human lung epithelial cancer cell lines. The physical properties of the samples were characterized by X-ray diffraction, Fourier transform infrared spectroscopy, high resolution scanning electron microscopy, high resolution transmission electron microscopy, N2 adsorption/desorption techniques and thermogravimetric analysis respectively. These metals modified hierarchical ZSM-5 zeolites showed considerable in-vitro anticancer efficiency towards human lung cancer (A549) cell lines through (3-(4, 5-dimethyl thiazolyl)-2, 5-diphenyl tetrazolium bromide) MTT assay. Among the all M (Cu, Ni)-modified ZSM-5 zeolites, 5% Cu-ZSM-5 zeolites showed higher potential cytotoxicity against A459 cell lines. The possible mechanism was explored from the fundamental signaling pathways of cell death by hierarchical metal modified ZSM-5 zeolites in A459 human lung epithelial cancer cell lines. Finally, our experimental results revealed that the organic template-free hierarchical pure and M (Cu, Ni)-modified ZSM-5 zeolites have significant anti-cancer mediating action by inducing oxidative stress to intercede DNA damage and can be considered as a potential applicant in the biomedical field. [Display omitted] •Synthesis of hierarchical pure and M (Cu, Ni)-modified ZSM-5 zeolites was achieved by a simple hydrothermal method.•It is a potential anti-cancer agent against A59 human lung cancer cell lines.•A possible mechanism for cytotoxic activity is discussed.