Molecular characterization and phylogenetic analysis of betasatellite molecules associated with okra yellow vein mosaic disease in Sri Lanka

Okra production in Sri Lanka has been severely affected by okra yellow vein mosaic disease (OYVMD), which is caused by begomoviruses and associated betasatellites. These betasatellite molecules commonly determine the development and severity of the disease. Therefore, knowledge about the genetic var...

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Bibliographic Details
Published in:Tropical plant pathology 2018-10, Vol.43 (5), p.468-472
Main Authors: Jeyaseelan, Tharmila C., Jeyaseelan, Emmanuel C., De Costa, Devika M., Shaw, Michael W.
Format: Article
Language:English
Subjects:
Abelmoschus esculentus
Amino acid sequence
Begomovirus
Biomedical and Life Sciences
Breeding
Conserved sequence
Deoxyribonucleic acid
DNA
Gene sequencing
Genetic analysis
Genetic relationship
Genetic variability
Genomes
Life Sciences
Mosaic disease
Nucleotide sequence
Okra
Phylogenetics
Phylogeny
Plant Pathology
Plant viruses
Proteins
Recombination
Short Communication
Viruses
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Summary:Okra production in Sri Lanka has been severely affected by okra yellow vein mosaic disease (OYVMD), which is caused by begomoviruses and associated betasatellites. These betasatellite molecules commonly determine the development and severity of the disease. Therefore, knowledge about the genetic variability of betasatellites associated with OYVMD could assist okra breeding programs in the selection of resistant varieties. The present study aimed to characterize the betasatellite DNA sequences associated with OYVMD in Sri Lanka and to determine their phylogenetic relationships. Betasatellite DNA of six virus isolates from widely separated geographical locations were sequenced and compared with already reported begomovirus betasatellites. The betasatellite molecules have features common to other betasatellite DNAs: a conserved nonanucleotide TAATATTAC, a coding sequence for the protein βC1, an adenine rich region and a satellite conserved region. Nucleotide diversity among the isolates was relatively low (π = 0.034). A recombination event was detected at a specific region in the genome of all isolates. The isolates shared >96% sequence identity with bhendi yellow vein betasatellites reported from India and phylogenetic analysis confirmed their genetic relationship.
ISSN:1983-2052
1982-5676
1983-2052
DOI:10.1007/s40858-018-0225-1