Isolation, amplification and characterization of foodborne pathogen disease bacteria gene for rapid kit test development

• Main Authors: Nurjayadi, M., Santoso, I., Kartika, I. R., Kurniadewi, F., Saamia, V., Sofihan, W., Nurkhasanah, D.
• Format: Conference Proceeding
• Language: English
• Subjects: Amplification; Bacteria; Criminal investigations; Developing countries; E coli; Electrophoresis; Food; Food contamination & poisoning; Genes; LDCs; Pathogens; Police; Polymerase chain reaction; Product safety; Salmonella
• ISSN: 0094-243X; 1551-7616
• DOI: 10.1063/1.4991181

There is a lot of public concern over food safety. Food-safety cases recently, including many food poisoning cases in both the developed and developing countries, considered to be the national security threats which involved police investigation. Quick and accurate detection methods are needed to handle the food poisoning cases with a big number of sufferers at the same time. Therefore, the research is aimed to develop a specific, sensitive, and rapid result molecular detection tool for foodborne pathogen bacteria. We, thus, propose genomic level approach with Polymerase Chain Reaction. The research has successfully produced a specific primer to perform amplification to fim-C S. typhi, E. coli, and pef Salmonella typhimurium genes. The electrophoresis result shows that amplification products are 95 base pairs, 121 base pairs, and 139 base pairs; and all three genes are in accordance with the size of the in silico to third genes bacteria. In conclusion, the research has been successfully designed a specific detection tool to three foodborne pathogen bacteria genes. Further stages test and the uses of Real-time PCR in the detection are still in the trial process for better detection method.