Isolation and characterization of serum extracellular vesicles (EVs) from Atlantic salmon infected with Piscirickettsia salmonis

Background: Secretion of extracellular vesicles (EVs) is a common feature of both eukaryotic and prokaryotic cells. Isolated EVs have been shown to contain different types of molecules, including proteins and nucleic acids and reported to be key players in intercellular communication. Little is know...

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Bibliographic Details
Published in:Journal of extracellular vesicles 2018-01, Vol.7, p.169-169
Main Authors: Lagos, Leidy, Tandberg, Julia, Kashulin-Bekkelund, Alexander, Colquhoun, Duncan, Sørum, Henning, Winther-Larsen, Hanne Cecilie
Format: Article
Language:English
Subjects:
Cell signaling
Disseminated infection
Extracellular vesicles
Fish
Flow cytometry
Histocompatibility
Immune response
Infections
Mass spectroscopy
Nanoparticles
Proteasomes
Proteins
Salmo salar
Salmon
Transmission electron microscopy
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Summary:Background: Secretion of extracellular vesicles (EVs) is a common feature of both eukaryotic and prokaryotic cells. Isolated EVs have been shown to contain different types of molecules, including proteins and nucleic acids and reported to be key players in intercellular communication. Little is known, however, of EV secretion in fish, and the effect of infection on EV release and content. In the present study, EVs were isolated from the serum of healthy and Piscirickettsia salmonisinfected Atlantic salmon in order to evaluate the effect of infection on EV secretion. P. salmonis is facultative intracellular bacterium that causes a systemic infection disease in farmed salmonids. Methods: EVs isolated from both infected and non-infected fish had and average diameter of 230-300 nm as confirmed by transmission electron microscopy, nanoparticle tracking analysis and flow cytometry. Results: Mass spectrometry identified 167 proteins in serum EVs from both groups of fish. Interestingly, 35 unique proteins were identified in serum EVs isolated from the fish infected with P. salmonis. These unique proteins included proteasomes subunits, granulins and major histocompatibility class I and II. Summary/Conclusion: Our results suggest that EV release could be part of a mechanism in which host stimulatory molecules are released from infected cells to promote an immune response.
ISSN:2001-3078