Scale‐up and economic analysis of the production of ligninolytic enzymes from a side‐stream of the organosolv process

BACKGROUND Organosolv pretreatment produces a hemicellulose‐rich stream with limited applications due to its toxicity. In this study, beech wood organosolv liquor was valorized through the production of ligninolytic enzymes, the extraction of ganoderic acid and the detoxification of this process str...

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Published in:Journal of chemical technology and biotechnology (1986) 2018-11, Vol.93 (11), p.3125-3134
Main Authors: Lú‐Chau, Thelmo A, Martínez‐Patiño, José C, Gullón, Beatriz, García‐Torreiro, María, Moreira, María T, Lema, Juan M, Eibes, Gemma
Format: Article
Language:English
Subjects:
Beech
Bioreactors
Biorefineries
Carbon sources
Cost analysis
Detoxification
Economic analysis
Economics
Enzymes
Feasibility studies
Fungi
Ganoderic acid
Ganoderma lucidum
Hemicellulose
Laccase
Ligninolytic enzymes
lignocellulose
Manganese
Manganese peroxidase
Mushrooms
Organic chemistry
organosolv fractionation
Peroxidase
Pharmacology
Pretreatment
Profitability
Rot
Toxicity
Viability
white‐rot fungi
Wood
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Summary:BACKGROUND Organosolv pretreatment produces a hemicellulose‐rich stream with limited applications due to its toxicity. In this study, beech wood organosolv liquor was valorized through the production of ligninolytic enzymes, the extraction of ganoderic acid and the detoxification of this process stream. RESULTS Laccase and manganese peroxidase (MnP) were produced in 30 L bioreactors by the white‐rot fungi Ganoderma lucidum and Irpex lacteus, respectively, using the hemicellulose‐rich liquor as carbon source. Both fungi caused a significant reduction in the hemicellulose stream toxicity (between 6.1 and 8.3 times lower). The extraction of ganoderic acid (24.3 mg g−1 of G. lucidum biomass) as a fungal metabolite with high pharmacological activity greatly increased the profitability of the process. Finally, the economic viability of the proposed valorization strategy was analyzed on the basis of the operational costs estimated for the process scale‐up. The specific costs of MnP and laccase production were €2.27 kU−1and €1.08 kU−1, respectively, which are 1109 and 78 times lower than the average price of commercially available enzymes. CONCLUSION It was found that the integration of the proposed valorization strategy into an organosolv‐based biorefinery would help to increase the economic feasibility of this pretreatment technology. © 2018 Society of Chemical Industry
ISSN:0268-2575
1097-4660
DOI:10.1002/jctb.5664