Single-walled carbon nanotubes affect the expression of genes associated with immune response in normal human astrocytes

• Published in: Toxicology in vitro 2018-10, Vol.52, p.122-130
• Main Authors: Minchenko, Oleksandr H., Tsymbal, Dariia O., Minchenko, Dmytro O., Prylutska, Svitlana V., Hnatiuk, Oksana S., Prylutskyy, Yuriy I., Tsierkezos, Nikos G., Ritter, Uwe
• Format: Article
• Language: English
• Subjects: Apoptosis; Astrocytes; Astrocytes - drug effects; Astrocytes - immunology; Astrocytes - metabolism; Biomedical materials; Carbon; Cell Line; Cell proliferation; Cell surface; Data processing; Deregulation; Drb1 protein; Gene expression; Gene Expression Regulation - drug effects; Gene Expression Regulation - immunology; Genes; Genomes; Glycoproteins; High temperature; High-Temperature Requirement A Serine Peptidase 1 - genetics; Histocompatibility antigen HLA; Histocompatibility Antigens Class I - genetics; HLA-DR alpha-Chains - genetics; HLA-DRA expression; Homology; Human behavior; Humans; Immune response; Immune system; Lamin Type B - genetics; Major histocompatibility complex; MicroRNAs - genetics; Nanotechnology; Nanotubes; Nanotubes, Carbon - toxicity; Normal human astrocytes; Nuclear Proteins - genetics; Pleckstrin; RNA, Messenger - metabolism; Single wall carbon nanotubes; Single-walled carbon nanotubes; Temperature requirements
• ISSN: 0887-2333; 1879-3177
• DOI: 10.1016/j.tiv.2018.06.011

The effect of single-walled carbon nanotubes (SWCNTs) on the expression of a subset of immune response, apoptosis and cell proliferation –associated genes was studied in normal human astrocytes (line NHA/TS). In the cells treated with SWCNTs (2, 10 and 50 ng/ml of medium for 24 h) we observed a strong dose-dependent down-regulation of the expression of a cell surface glycoproteins HLA-DRA (major histocompatibility complex, class II, DR alpha) and HLA-DRB1. At the same time, the expression of HLA-F (major histocompatibility complex, class I, F), LMNB1 (lamin B1), and HTRA1 (high temperature requirement A1) genes as well as the level of miR-190b and miR-7 was up-regulated in NHA/TS subjected to different concentrations of SWCNTs. After 24 h of treatment with SWCNTs we detected a dose-dependent suppression of PHLDA2 (pleckstrin homology-like domain, family A, member 2) gene expression in these cells. Obtained data show that SWCNTs may affect an immune response, in particular through suppression of HLA-DRA and HLA-DRB1 gene expressions and that miR-190b and miR-7 possibly participated in this suppression. Deregulation of lamin B1 expression indicates the possibility of alterations in genome stability following treatment of astrocytes with SWCNTs. Thus, more caution is needed in biomedical application of SWCNTs. •SWCNTs affect immune response through suppression of cell surface glycoproteins HLA-DR•MicroRNA miR-7 and miR-190b are upregulated by SWCNTs and suppress immune response•Deregulation of lamin B1 expression indicates genome stability alterations in cells treated by SWCNTs