Position, orientation, and relative quantum yield ratio determination of fluorescent nanoemitters via combined laser scanning microscopy and polarization measurements

We present a universal method of determining the position, 3D orientation, andrelative quantum yield ratio (RQYR) of fluorescent nanoemitters (ZnS coated CdSe quantumdots) in a glass slide by combining laser scanning microscopy (LSM) and polarizationmeasurements. The quantum dots were located throug...

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Bibliographic Details
Published in:Optical materials express 2018-05, Vol.8 (5), p.1290
Main Authors: Dasallas, Lean L., Jaculbia, Rafael B., Balois, Maria Vanessa, Garcia, Wilson O., Hayazawa, Norihiko
Format: Article
Language:English
Subjects:
Dipole moments
Fluorescence
Microscopy
Polarization
Position measurement
Quantum dots
Scanning microscopy
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Summary:We present a universal method of determining the position, 3D orientation, andrelative quantum yield ratio (RQYR) of fluorescent nanoemitters (ZnS coated CdSe quantumdots) in a glass slide by combining laser scanning microscopy (LSM) and polarizationmeasurements. The quantum dots were located through LSM intensity maps using azimuthal,radial, and linear incident polarizations. LSM imaging was not sufficient to determine theorientation of the quantum dots due to the isotropic absorption dipole moment. The 3Dorientation was obtained through polarization measurement. By combining LSM andpolarization measurements, the RQYR of a single molecule was evaluated, allowing us tocompare the quantum yield of the nanoemitters.
ISSN:2159-3930
2159-3930
DOI:10.1364/OME.8.001290