The effect of organic solvents and other parameters on trypsin-catalyzed hydrolysis of N-alpha-benzoyl-arginine-p-nitroanilide

Trypsin is a serine protease that has both amidase and esterase activity. Its mechanism of action is based on a nucleophilic catalysis with acid-base assistance and depends on the presence of a catalytic triad.

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Bibliographic Details
Published in:Journal of chemical education 2001-11, Vol.78 (11), p.1535
Main Authors: Correia, L C, Bocewicz, A C, Esteves, S A, Pontes, M G
Format: Article
Language:English
Subjects:
Amidase
Catalysis
Catalysts
Chemistry
Enzymes
Organic solvents
Solvents
Trypsin
Online Access:Get full text
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Description
Summary:Trypsin is a serine protease that has both amidase and esterase activity. Its mechanism of action is based on a nucleophilic catalysis with acid-base assistance and depends on the presence of a catalytic triad.
ISSN:0021-9584
1938-1328