Aberrant Amygdala-Dependent Cued Fear Memory in Na+/Ca2+ Exchanger 1 Heterozygous Mice

• Published in: Molecular neurobiology 2019-06, Vol.56 (6), p.4381-4394
• Main Authors: Moriguchi, Shigeki, Kita, Satomi, Inagaki, Ryo, Yabuki, Yasushi, Sasaki, Yuzuru, Ishikawa, Shun, Sakagami, Hiroyuki, Iwamoto, Takahiro, Fukunaga, Kohji
• Format: Article
• Language: English
• Subjects: Amygdala; Biomedical and Life Sciences; Biomedicine; c-Fos protein; Ca2+/calmodulin-dependent protein kinase II; Ca2+/calmodulin-dependent protein kinase IV; Calcium; Cell Biology; Electrochemistry; Isoforms; Long-term potentiation; Memory; Mice; Na+/Ca2+ exchanger; NCX1 protein; Neurobiology; Neurology; Neurosciences; Rodents
• ISSN: 0893-7648; 1559-1182
• DOI: 10.1007/s12035-018-1384-2

Na + /Ca 2+ exchangers (NCXs) are mainly expressed in the plasma membrane and exchange one Ca 2+ for three Na + , depending on the electrochemical gradients across the plasma membrane. NCXs have three isoforms, NCX1–3, encoded by distinct genes in mammals. Here, we report that heterozygous mice lacking NCX1 (NCX1 +/− ) exhibit impaired amygdala-dependent cued fear memory. NCX1 +/− mice showed significant impairment in fear-related behaviors measured with the elevated-plus maze, light-dark, open-field, and marble-burying tasks. In addition, NCX1 +/− mice showed abnormality in cued fear memory but not in contextual fear memory in a fear-conditioning task. In immunohistochemical analyses, NCX1 +/− mice had significantly increased number of c-Fos-positive cells in the lateral amygdala (LA) but not in the central amygdala following fear-related tone stimuli. c-Fos expression peaked at 1 h. In concordance with the aberrant fear-related behaviors in NCX1 +/− mice, enhanced long-term potentiation was also observed in the LA of these mice. Furthermore, enhancement of CaMKII or CaMKIV activity in the LA was observed in NCX1 +/− mice by immunoblot analyses. In contrast, CaMKII +/− but not CaMKIV −/− mice insufficiently exhibited tone-induced cued fear memory and there was no increase in the number of c-Fos-positive cells in the LA. Altogether, the increased CaMKII activity and consequent c-Fos expression likely account for the dysregulation of amygdala-dependent cued fear memory in NCX1 +/− mice.