Preparation of Orthosiphon stamineus enriched-extracts and evaluation of their free radical scavenging activity

Orthosiphon stamineus is one of the important herbal plants used in folk medicine to cure variety of diseases. Three compounds namely rosmarinic acid (RA), sinensetin (SEN) and eupatorin (EUP) were identified as the bioactive markers. However, a standardized extraction method for the preparation of...

Full description

Saved in:
Bibliographic Details
Main Authors: Mansor, Che Nurul Ain Nadirah Che, Latip, Jalifah, Markom, Masturah
Format: Conference Proceeding
Language:English
Subjects:
Biological activity
Biomarkers
Enrichment
Ethanol
Free radicals
Maceration
Rosmarinic acid
Scavenging
Thin layer chromatography
Toluene
Vitamin C
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Orthosiphon stamineus is one of the important herbal plants used in folk medicine to cure variety of diseases. Three compounds namely rosmarinic acid (RA), sinensetin (SEN) and eupatorin (EUP) were identified as the bioactive markers. However, a standardized extraction method for the preparation of O. stamineus extract enriched with the bioactive compounds was still undiscovered. Thus, this study aims to establish the optimal extraction method that can be used to prepare the enriched extract with anti-oxidant property. Maceration, reflux and Soxhlet were the extraction methods employed, with ethanol, 50% (v/v) aqueous ethanol and water were chosen as the solvents. Each extracts were evaluated for their biomarker contents (RA, SEN and EUP) and anti-oxidant capacity using thin layer chromatography (TLC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay respectively. Among the three extraction methods employed, the highest total extraction yield was obtained from reflux (72.73%) followed by Soxhlet (62.51%) and maceration (37.78%). Although all extracts found to contain the three biomarkers via TLC visualization analysis, there was variation in the extracts’ anti-oxidant capacity, ranging from 6.17% to 72.97%. The variation was expected to be due to the difference in the quantity of the biomarkers in each extract. Furthermore, the anti-oxidative potency of the RA was found comparable to natural anti-oxidant vitamin C, and higher than the synthetic anti-oxidant butylated hydroxyl toluene (BHT). These preliminary results may serve as a starting point towards the preparation of standardized bioactive O. stamineus extract.
ISSN:0094-243X
1551-7616
DOI:10.1063/1.4966751