Quantitative Determination of HIV-Antiviral Agent using Complexing Reagent in Bulk Material and Formulations

A sensitive visible spectrophotometric method was developed for quantitative determination of Antiviral [Abacavir sulphate (AVS)] agent against HIV in bulk material and dosage forms. An extraction spectrophotometric method for the assay of abacavir sulphate is based on the formation of colored co-or...

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Bibliographic Details
Published in:Oriental journal of chemistry 2014, Vol.30 (1), p.333-339
Main Authors: Sastry, Thuttagunta, Ramakrishna, Karipeddi, Lavanya, Kotturu, Prasad, Kallempudi
Format: Article
Language:English
Subjects:
Absorptivity
Calibration
Cobalt
Formulations
Laboratories
Methods
Reagents
Solvents
Spectrophotometry
Thiocyanates
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Summary:A sensitive visible spectrophotometric method was developed for quantitative determination of Antiviral [Abacavir sulphate (AVS)] agent against HIV in bulk material and dosage forms. An extraction spectrophotometric method for the assay of abacavir sulphate is based on the formation of colored co-ordination complex with cobalt thiocyanate (CTC) which can be extractable in nitrobenzene (lmax=620 nm) is described. Beer's law limits and Molar absorptivity values were found to be 10 – 50 mg mL-1 and 9.58 x 103 L mol-1cm-1 respectively. The limit of detection and limit of quantification values are found to be 3.07 x 10-1 µg mL-1 and 9.3 x 10-1 µg mL-1 respectively. Precision and accuracy of the developed method was evaluated. The results are validated statistically and compared with reported methods.
ISSN:0970-020X
2231-5039
DOI:10.13005/ojc/300144