Characterisation of immunodominant protein encoded by the F1L gene of orf virus strains isolated in Italy: Brief Report

We analysed the molecular properties of the immunodominant protein of different orf virus strains isolated in Italy. The F1L encoding genes and the deduced amino acid sequences of all strains were determined and compared, and they showed several mutations. Structural analysis was carried out in orde...

Full description

Saved in:
Bibliographic Details
Published in:Archives of virology 2002-09, Vol.147 (10), p.1989-1995
Main Authors: Scagliarini, A., Ciulli, S., Battilani, M., Jacoboni, I., Montesi, F., Casadio, R., Prosperi, S.
Format: Article
Language:English
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page 1995
container_issue 10
container_start_page 1989
container_title Archives of virology
container_volume 147
creator Scagliarini, A.
Ciulli, S.
Battilani, M.
Jacoboni, I.
Montesi, F.
Casadio, R.
Prosperi, S.
description We analysed the molecular properties of the immunodominant protein of different orf virus strains isolated in Italy. The F1L encoding genes and the deduced amino acid sequences of all strains were determined and compared, and they showed several mutations. Structural analysis was carried out in order to assess the influence of amino acid variations on protein structure demonstrating a conservation of the secondary structure. Western blot analysis and immunogold electron microscopy showed that all orf virus strains were antigenically identical. The results of our study confirmed the immunogenicity of the F1L protein; furthermore, our data suggest a possible involvement of the protein in the virus cycle.
doi_str_mv 10.1007/s00705-002-0850-2
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_212401329</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>708224681</sourcerecordid><originalsourceid>FETCH-LOGICAL-c699-df0e4a4826134a32d15b610dc95c7997382119151cabc529bce8aea74b3005a23</originalsourceid><addsrcrecordid>eNotkE1rAyEQhqW00DTtD-hNercddd3VYwn9CAR6yV1c120MWU3VLeTf15BeZmB43pnhQeiRwjMF6F5yLSAIACMgBRB2hRa04YzITslrtAAODZEtyFt0l_MeoA64WCC_2plkbHHJZ1N8DDiO2E_THOIQJx9MKPiYYnE-YBdsHNyA-xMuO4ff6QZ_u-DOiZhG_OvTnHEuyfiQsc_xYEqla3BdzOF0j25Gc8ju4b8v0fb9bbv6JJuvj_XqdUNsqxQZRnCNaSRrKW8MZwMVfUthsErYTqmOS0apooJa01vBVG-dNM50Tc8BhGF8iZ4ua-vXP7PLRe_jnEK9qBllDVDOVIXoBbIp5pzcqI_JTyadNAV99qkvPnX1qc8-NeN_gqRofQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>212401329</pqid></control><display><type>article</type><title>Characterisation of immunodominant protein encoded by the F1L gene of orf virus strains isolated in Italy: Brief Report</title><source>Springer Link</source><creator>Scagliarini, A. ; Ciulli, S. ; Battilani, M. ; Jacoboni, I. ; Montesi, F. ; Casadio, R. ; Prosperi, S.</creator><creatorcontrib>Scagliarini, A. ; Ciulli, S. ; Battilani, M. ; Jacoboni, I. ; Montesi, F. ; Casadio, R. ; Prosperi, S.</creatorcontrib><description>We analysed the molecular properties of the immunodominant protein of different orf virus strains isolated in Italy. The F1L encoding genes and the deduced amino acid sequences of all strains were determined and compared, and they showed several mutations. Structural analysis was carried out in order to assess the influence of amino acid variations on protein structure demonstrating a conservation of the secondary structure. Western blot analysis and immunogold electron microscopy showed that all orf virus strains were antigenically identical. The results of our study confirmed the immunogenicity of the F1L protein; furthermore, our data suggest a possible involvement of the protein in the virus cycle.</description><identifier>ISSN: 0304-8608</identifier><identifier>EISSN: 1432-8798</identifier><identifier>DOI: 10.1007/s00705-002-0850-2</identifier><language>eng</language><publisher>Wien: Springer Nature B.V</publisher><ispartof>Archives of virology, 2002-09, Vol.147 (10), p.1989-1995</ispartof><rights>Copyright Springer-Verlag 2002</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids></links><search><creatorcontrib>Scagliarini, A.</creatorcontrib><creatorcontrib>Ciulli, S.</creatorcontrib><creatorcontrib>Battilani, M.</creatorcontrib><creatorcontrib>Jacoboni, I.</creatorcontrib><creatorcontrib>Montesi, F.</creatorcontrib><creatorcontrib>Casadio, R.</creatorcontrib><creatorcontrib>Prosperi, S.</creatorcontrib><title>Characterisation of immunodominant protein encoded by the F1L gene of orf virus strains isolated in Italy: Brief Report</title><title>Archives of virology</title><description>We analysed the molecular properties of the immunodominant protein of different orf virus strains isolated in Italy. The F1L encoding genes and the deduced amino acid sequences of all strains were determined and compared, and they showed several mutations. Structural analysis was carried out in order to assess the influence of amino acid variations on protein structure demonstrating a conservation of the secondary structure. Western blot analysis and immunogold electron microscopy showed that all orf virus strains were antigenically identical. The results of our study confirmed the immunogenicity of the F1L protein; furthermore, our data suggest a possible involvement of the protein in the virus cycle.</description><issn>0304-8608</issn><issn>1432-8798</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><recordid>eNotkE1rAyEQhqW00DTtD-hNercddd3VYwn9CAR6yV1c120MWU3VLeTf15BeZmB43pnhQeiRwjMF6F5yLSAIACMgBRB2hRa04YzITslrtAAODZEtyFt0l_MeoA64WCC_2plkbHHJZ1N8DDiO2E_THOIQJx9MKPiYYnE-YBdsHNyA-xMuO4ff6QZ_u-DOiZhG_OvTnHEuyfiQsc_xYEqla3BdzOF0j25Gc8ju4b8v0fb9bbv6JJuvj_XqdUNsqxQZRnCNaSRrKW8MZwMVfUthsErYTqmOS0apooJa01vBVG-dNM50Tc8BhGF8iZ4ua-vXP7PLRe_jnEK9qBllDVDOVIXoBbIp5pzcqI_JTyadNAV99qkvPnX1qc8-NeN_gqRofQ</recordid><startdate>200209</startdate><enddate>200209</enddate><creator>Scagliarini, A.</creator><creator>Ciulli, S.</creator><creator>Battilani, M.</creator><creator>Jacoboni, I.</creator><creator>Montesi, F.</creator><creator>Casadio, R.</creator><creator>Prosperi, S.</creator><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope></search><sort><creationdate>200209</creationdate><title>Characterisation of immunodominant protein encoded by the F1L gene of orf virus strains isolated in Italy</title><author>Scagliarini, A. ; Ciulli, S. ; Battilani, M. ; Jacoboni, I. ; Montesi, F. ; Casadio, R. ; Prosperi, S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c699-df0e4a4826134a32d15b610dc95c7997382119151cabc529bce8aea74b3005a23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Scagliarini, A.</creatorcontrib><creatorcontrib>Ciulli, S.</creatorcontrib><creatorcontrib>Battilani, M.</creatorcontrib><creatorcontrib>Jacoboni, I.</creatorcontrib><creatorcontrib>Montesi, F.</creatorcontrib><creatorcontrib>Casadio, R.</creatorcontrib><creatorcontrib>Prosperi, S.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health &amp; Medical Complete (ProQuest Database)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><jtitle>Archives of virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Scagliarini, A.</au><au>Ciulli, S.</au><au>Battilani, M.</au><au>Jacoboni, I.</au><au>Montesi, F.</au><au>Casadio, R.</au><au>Prosperi, S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterisation of immunodominant protein encoded by the F1L gene of orf virus strains isolated in Italy: Brief Report</atitle><jtitle>Archives of virology</jtitle><date>2002-09</date><risdate>2002</risdate><volume>147</volume><issue>10</issue><spage>1989</spage><epage>1995</epage><pages>1989-1995</pages><issn>0304-8608</issn><eissn>1432-8798</eissn><abstract>We analysed the molecular properties of the immunodominant protein of different orf virus strains isolated in Italy. The F1L encoding genes and the deduced amino acid sequences of all strains were determined and compared, and they showed several mutations. Structural analysis was carried out in order to assess the influence of amino acid variations on protein structure demonstrating a conservation of the secondary structure. Western blot analysis and immunogold electron microscopy showed that all orf virus strains were antigenically identical. The results of our study confirmed the immunogenicity of the F1L protein; furthermore, our data suggest a possible involvement of the protein in the virus cycle.</abstract><cop>Wien</cop><pub>Springer Nature B.V</pub><doi>10.1007/s00705-002-0850-2</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0304-8608
ispartof Archives of virology, 2002-09, Vol.147 (10), p.1989-1995
issn 0304-8608
1432-8798
language eng
recordid cdi_proquest_journals_212401329
source Springer Link
title Characterisation of immunodominant protein encoded by the F1L gene of orf virus strains isolated in Italy: Brief Report
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-26T08%3A36%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Characterisation%20of%20immunodominant%20protein%20encoded%20by%20the%20F1L%20gene%20of%20orf%20virus%20strains%20isolated%20in%20Italy:%20Brief%20Report&rft.jtitle=Archives%20of%20virology&rft.au=Scagliarini,%20A.&rft.date=2002-09&rft.volume=147&rft.issue=10&rft.spage=1989&rft.epage=1995&rft.pages=1989-1995&rft.issn=0304-8608&rft.eissn=1432-8798&rft_id=info:doi/10.1007/s00705-002-0850-2&rft_dat=%3Cproquest_cross%3E708224681%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c699-df0e4a4826134a32d15b610dc95c7997382119151cabc529bce8aea74b3005a23%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=212401329&rft_id=info:pmid/&rfr_iscdi=true