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Molecular cloning and characterization of alpha - galactosidase gene from Glaciozyma antarctica

Psychrophilic enzymes are proteins produced by psychrophilic organisms which recently are the limelight for industrial applications. A gene encoding α-galactosidase from a psychrophilic yeast, Glaciozyma antarctica PI12 which belongs to glycoside hydrolase family 27, was isolated and analyzed using...

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Bibliographic Details
Main Authors: Moheer Reyad Qaed Al, Bakar Farah Diba Abu, Murad Abdul Munir Abdul
Format: Conference Proceeding
Language:English
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Summary:Psychrophilic enzymes are proteins produced by psychrophilic organisms which recently are the limelight for industrial applications. A gene encoding α-galactosidase from a psychrophilic yeast, Glaciozyma antarctica PI12 which belongs to glycoside hydrolase family 27, was isolated and analyzed using several bioinformatic tools. The cDNA of the gene with the size of 1,404-bp encodes a protein with 467 amino acid residues. Predicted molecular weight of protein was 48.59 kDa and hence we name the gene encoding α-galactosidase as GAL48. We found that the predicted protein sequences possessed signal peptide sequence and are highly conserved among other fungal α-galactosidase.
ISSN:0094-243X
1551-7616
DOI:10.1063/1.4931247