Lycopene influences osteoblast functional activity and prevents femur bone loss in female rats submitted to an experimental model of osteoporosis

• Published in: Journal of bone and mineral metabolism 2019-07, Vol.37 (4), p.658-667
• Main Authors: Oliveira, Gustavo Ribeiro, Vargas-Sanchez, Paula Katherine, Fernandes, Roger Rodrigo, Ricoldi, Milla Sprone Tavares, Semeghini, Mayara Sgarbi, Pitol, Dimitrius Leonardo, de Sousa, Luiz Gustavo, Siessere, Selma, Bombonato-Prado, Karina Fittipaldi
• Format: Article
• Language: English
• Subjects: Adipocytes - drug effects; Adipocytes - metabolism; Alkaline phosphatase; Alkaline Phosphatase - metabolism; Animals; Antioxidants; Bone Density - drug effects; Bone loss; Bone marrow; Bone Marrow Cells - drug effects; Bone Marrow Cells - metabolism; Bone Matrix - drug effects; Bone Matrix - metabolism; Bone remodeling; Bone resorption; Bone Resorption - drug therapy; Bone Resorption - pathology; Bone Resorption - physiopathology; Bone Resorption - prevention & control; Bone turnover; Calcification, Physiologic - drug effects; Cancellous bone; Cancellous Bone - drug effects; Cancellous Bone - pathology; Cbfa-1 protein; Cell culture; Cell proliferation; Cell Proliferation - drug effects; Disease Models, Animal; Epiphysis; Female; Femur; Femur - drug effects; Femur - pathology; Gene Expression Regulation - drug effects; Lycopene; Lycopene - pharmacology; Lycopene - therapeutic use; Medicine; Medicine & Public Health; Metabolic Diseases; Metabolism; Mineralization; Nutrients; Original Article; Orthopedics; Osteoblasts; Osteoblasts - drug effects; Osteoblasts - metabolism; Osteogenesis - drug effects; Osteoporosis; Osteoporosis - drug therapy; Osteoporosis - pathology; Osteoporosis - physiopathology; Ovariectomy; Oxidative stress; Rats, Wistar; RNA, Messenger - genetics; RNA, Messenger - metabolism; Rodents; Surgery
• ISSN: 0914-8779; 1435-5604
• DOI: 10.1007/s00774-018-0970-8

Antioxidant properties of several nutrients may influence bone metabolism, affording protection against damaging effects caused by oxidative stress. Thus, we hypothesized that lycopene may benefit bone tissue metabolism and functional activity of osteoblastic cells from bone marrow of osteoporotic female rats. Wistar rats were ovariectomized and paired with sham animals. In vitro evaluations were performed after 60 days of surgery, when cells were cultured in osteogenic medium and divided in control (C), ovariectomized (OVX) and ovariectomized + 1 μmol/L lycopene (OVXL) groups. Besides, in vivo studies were carried out to evaluate femur bone remodeling by histological and histomorphometric analyses after daily intake of 10 mg/kg of lycopene for 30 and 60 days after ovariectomy. Cell proliferation was significantly higher in OVX and OVXL groups after 10 days of culture. Alkaline phosphatase activity (ALP) was higher in OVXL group in later periods of cell culture, whereas its in situ detection was higher for this group in all experimental periods; nevertheless, mineralization did not show significant differences among the groups. There was a significant upregulation of genes Sp7 , Runx2 and Bsp after 3 days and genes Runx2 and Bglap after 10 days from OVXL when compared to OVX. In vivo results demonstrated that daily intake of 10 mg/kg of lycopene for 60 days decreased bone loss in femur epiphysis in ovariectomized rats by maintaining trabecular bone similar to controls. Data obtained suggest that lycopene might benefit the functional activity of osteoblastic cells from ovariectomized rats, as well as avoid further bone resorption.