A tripod anchor offers improved robustness of peptide-based electrochemical biosensors

•A methylene blue-tagged peptide-based probe endowed with a triple anchor was generated for the first time.•The tripod anchor probe was used to produced SAMs with enhanced long-term, thermal and chemical stability.•The tripod anchor probe showed efficient electron transfer and protease detection. Pe...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2018-11, Vol.274, p.662-667
Main Authors: Staderini, Matteo, González-Fernández, Eva, Murray, Alan F., Mount, Andrew R., Bradley, Mark
Format: Article
Language:English
Subjects:
Anchors
Biological properties
Biosensors
Data analysis
Detection
Electrodes
Organic chemistry
Peptide-based probe
Peptides
Protease detection
Proteases
SAMs stability
Self-assembled monolayers
Self-assembly
Stability analysis
Substrates
Tripod anchor
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Summary:•A methylene blue-tagged peptide-based probe endowed with a triple anchor was generated for the first time.•The tripod anchor probe was used to produced SAMs with enhanced long-term, thermal and chemical stability.•The tripod anchor probe showed efficient electron transfer and protease detection. Peptide-based electrochemical biosensors have proven to be powerful sensing strategies for the detection of proteases and offer a highly versatile system, as simple tuning of the immobilised substrate peptide leads to a new sensing platform. The most common immobilisation strategy of peptides onto an electrode surface is via a self-assembled monolayer (SAM) through a thiol group that can be readily chemically incorporated in the target peptide. However, the successful application of these platforms in complex biological samples can be frustrated by the lack of stability of the peptide-based SAM, that can lead to false positives and limited shelf-life. Herein, we investigated the stability of a peptide-based electrochemical platform endowed with a single or a tribranched thiol group for attaching onto the electrode surface. Side-by-side comparison demonstrated that the tripod anchor generated a highly robust peptide-based electrochemical biosensor that showed improved stability when compared to the monoanchor analogue, simplifying data analysis and interpretation, while showing efficient protease detection and similar sensing capabilities.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2018.07.100