LC–MS/MS Determination of Catecholamines in Urine Using FMOC-Cl Derivatization on Solid-Phase Extraction Cartridge

A method for the determination of catecholamine derivatives in human urine is proposed that includes the derivatization of target compounds on a solid-phase extraction cartridge and determination of the analytes by a UHPLC method with tandem mass-spectrometric detection. 9-Fluorenyl-methoxycarbonyl...

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Bibliographic Details
Published in:Chromatographia 2018-11, Vol.81 (11), p.1487-1494
Main Authors: Azaryan, A., Ligor, T., Buszewski, B., Temerdashev, A., Dmitrieva, E., Gashimova, E.
Format: Article
Language:English
Subjects:
Analytical Chemistry
Catecholamine
Chemistry
Chemistry and Materials Science
Chromatography
Dopamine
Ions
Laboratory Medicine
Original
Pharmacy
Proteomics
Solid phases
Spectrometry
Urine
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Summary:A method for the determination of catecholamine derivatives in human urine is proposed that includes the derivatization of target compounds on a solid-phase extraction cartridge and determination of the analytes by a UHPLC method with tandem mass-spectrometric detection. 9-Fluorenyl-methoxycarbonyl chloride was used as the derivatization agent. The limits of detection for the analytes were 2.5 ng mL −1 for 9-fluorenyl-methoxycarbonyl-adrenaline, 5 ng mL −1 for 9-fluorenyl-methoxycarbonyl-octopamine, and 25 ng mL −1 for 9-fluorenyl-methoxycarbonyl-dopamine. The proposed procedure was tested on real samples obtained from volunteers.
ISSN:0009-5893
1612-1112
DOI:10.1007/s10337-018-3610-5