AFLP-based genetic diversity assessment among wheat cultivars from the Pacific Northwest

Assessing genetic diversity among adapted, elite germplasm may expedite crop improvement. The objectives of this study were to (i) assess genetic diversity among a representative sample of spring and winter wheat (Triticum aestivum L.) cultivars adapted to dryland production in the Pacific Northwest...

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Bibliographic Details
Published in:Crop science 1998-09, Vol.38 (5), p.1261-1271
Main Authors: Barrett, B.A. (Washington State University, Pullman, WA.), Kidwell, K.K
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
AMPLIFIED FRAGMENT LENGTH POLYMORPHISM
Arid zones
ARIDOCULTURE
Biological and medical sciences
Botany
CLADISTICS
Crop improvement
Cultivars
CULTIVO EN TIERRAS ARIDAS
DRY FARMING
Enzymes
Fundamental and applied biological sciences. Psychology
Generalities. Genetics. Plant material
Genetic aspects
Genetic diversity
GENETIC POLYMORPHISM
Genetic polymorphisms
Genetic research
Genetic resources, diversity
GENETIC VARIATION
Genetics
Genetics and breeding of economic plants
GERMOPLASMA
GERMPLASM
HABITOS DE CRECIMIENTO
HIVER
INVIERNO
Methods
Methylation
NUCLEOTIDE SEQUENCE
PLANT HABIT
Plant material
POLIMORFISMO GENETICO
POLYMORPHISME GENETIQUE
PORT DE LA PLANTE
PRIMAVERA
PRINTEMPS
SECUENCIA NUCLEOTIDICA
SEQUENCE NUCLEOTIDIQUE
SPRING
SPRING AND WINTER HABIT
TRITICUM AESTIVUM
VARIACION GENETICA
VARIATION GENETIQUE
VARIEDADES
VARIETE
VARIETIES
Wheat
WINTER
Winter wheat
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Description
Summary:Assessing genetic diversity among adapted, elite germplasm may expedite crop improvement. The objectives of this study were to (i) assess genetic diversity among a representative sample of spring and winter wheat (Triticum aestivum L.) cultivars adapted to dryland production in the Pacific Northwest by amplified fragment length polymorphisms (AFLPs), and (ii) compare genetic diversity estimates (GDEs) generated by methylation sensitive and methylation insensitive restriction enzymes for AFLP analyses. Fifty-tour cultivars and two diploid relatives were included in the study. Sixteen AFLP primer pairs detected 229 polymorphic bands. Mean GDEs (0.51 and 0.58, respectively) detected by PstI:MseI (methylation sensitive) and EcoRI:MseI (methylation insensitive) AFLP analyses were significantly different (P 0.0001), indicating greater diversity was detected for methylated sequences. Mean GDEs, based on data from both restriction enzyme combinations, were highest (0.58) for spring vs. winter type pairwise comparisons, intermediate (0.53) within winter type, and lowest (0.49) within spring type. Cluster analysis, ordination analysis, and the analysis of molecular variance suggested genetic diversity among cultivars was hierarchically arranged as cultivars nested within market class, and market classes nested within growth habit. AFLP analysis is an efficient technology for assessing genetic diversity among wheat cultivars
ISSN:0011-183X
1435-0653
DOI:10.2135/cropsci1998.0011183X003800050025x