beta-1,3-glucanase and resistance to Aspergillus flavus infection in maize

• Published in: Crop science 1998-09, Vol.38 (5), p.1255
• Main Authors: Lozovaya, V.V. (University of Illinois, Urbana, IL.), Waranyuwat, A, Widholm, J.M
• Format: Article
• Language: English
• Subjects: ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; AFLATOXINAS; AFLATOXINE; AFLATOXINS; ASPERGILLUS FLAVUS; BETA GLUCANASA; BETA GLUCANASE; CAL; CALLO; CALLOSE; CALLUS; CELL WALLS; Corn; CRECIMIENTO; CROISSANCE; CULTIVO DE TEJIDOS; CULTURE DE TISSU; CULTURE MEDIA; DISEASE RESISTANCE; Disease susceptibility; EMBRIOGENESIS SOMATICA; EMBRYOGENESE SOMATIQUE; Enzymes; ENZYMIC ACTIVITY; EXPERIMENTACION IN VIVO; EXPERIMENTAL INFECTION; EXPERIMENTATION IN VIVO; Fungal diseases of plants; Fungi; Genetic aspects; GENETIC RESISTANCE; Genetics; GENOTIPOS; GENOTYPE; GENOTYPES; GRAINE; GROWTH; IN VIVO EXPERIMENTATION; INFECCION EXPERIMENTAL; INFECTION EXPERIMENTALE; INHIBICION; INHIBITION; MAIS; MAIZ; MAIZE; MAIZE EARS; MEDIO DE CULTIVO; MILIEU DE CULTURE; PARED CELULAR; PAROI CELLULAIRE; Plant diseases; POLISACARIDOS; POLYHOLOSIDE; POLYSACCHARIDES; RESISTANCE AUX MALADIES; RESISTANCE GENETIQUE; RESISTENCIA A LA ENFERMEDAD; RESISTENCIA GENETICA; SEEDS; SEMILLA; SOMATIC EMBRYOGENESIS; TISSUE CULTURE; ZEA MAYS
• ISSN: 0011-183X; 1435-0653
• DOI: 10.2135/cropsci1998.0011183X003800050024x

Studies of the activity levels of the fungal cell wall degradative enzyme, beta-1,3-glucanase, were carried out with Zea mays L. embryogenic callus cultures and kernels of genotypes with different abilities to resist aflatoxin formation on the ears. Dual-culture experiments of the aflatoxin forming fungus, Aspergillus flavus Link: Fr. with maize callus, demonstrated that the growth of A. flavus on plates was inhibited more by callus of a resistant genotype (Tex 6 x Mo17 denoted TXM) than by a sensitive genotype (Pa91). The inhibition correlated with the activity levels of beta-1,3-glucanase in the callus and in the culture medium. The presence of the fungus caused an increase in enzyme activity in TXM but not in Pa91 callus. Direct treatment of callus of both genotypes with A. flavus spores caused the amount of callose to increase within 30 min. An elevated beta-1,3-glucanase activity in maize kernels was also correlated with lower A. flavus infection observed in the resistant genotype (Tex 6) compared with a susceptible one (B73). Fungal inoculation of the ears increased the enzyme activities both in kernels in contact with and not in contact with the fungus. These studies indicate that beta-1,3-glucanase activity may have a role in the inhibition of growth of the A. flavus fungus, that maize embryogenic callus and kernels do respond to the presence of this nonpathogenic fungus, and that these systems might be useful in further plant-fungal interaction studies