Biological and anticancer properties of Inonotus obliquus extracts

[Display omitted] •I. obliquus increased antioxidative enzymes expression in the fibroblasts cells.•I. obliquus decreased enzymes expression in the colorectal adenocarcinoma cells.•I. obliquus decreased reactive oxygen species production in the fibroblasts cells.•I. obliquus increased reactive oxyge...

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Bibliographic Details
Published in:Process biochemistry (1991) 2018-10, Vol.73, p.180-187
Main Authors: Szychowski, Konrad A., Rybczyńska-Tkaczyk, Kamila, Tobiasz, Jakub, Yelnytska-Stawasz, Viktoriya, Pomianek, Tadeusz, Gmiński, Jan
Format: Article
Language:English
Subjects:
Adenocarcinoma
Anticancer
Anticancer properties
Antioxidant
Antiproliferative
Aqueous chemistry
Biochemistry
Biological properties
Cardiovascular diseases
Catalase
Cell culture
Cytotoxicity
Diabetes mellitus
Ethanol
Extraction processes
Free radicals
Gene expression
Inonotus obliquus
Medical treatment
Methanol
Reactive oxygen species
Scavenging
Skin
Solid solutions
Solution chemistry
Superoxide dismutase
Xanthine oxidase
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Summary:[Display omitted] •I. obliquus increased antioxidative enzymes expression in the fibroblasts cells.•I. obliquus decreased enzymes expression in the colorectal adenocarcinoma cells.•I. obliquus decreased reactive oxygen species production in the fibroblasts cells.•I. obliquus increased reactive oxygen species production in the carcinoma cells. Inonotus obliquus (IO) has traditionally been used in folk medicine in the treatment of gastrointestinal cancer, cardiovascular disease and diabetes. The aim of our study was to evaluate the biological and metabolic properties of IO extracts. Free radical scavenging properties, inhibition of the activity of xanthine oxidase (XOi), induction of reactive oxygen species (ROS) production, cell viability and expression of superoxide dismutase (SOD1), catalase (CAT) and KI67 mRNA in the human colorectal adenocarcinoma (Caco-2) and normal human skin fibroblast (BJ) cell lines were measured. It was found that 80% ethanol extract of IO exhibited the highest properties inhibiting the activity of XO and radical scavenging activities. PBS, ethanol and methanol extracts of IO increased ROS production in the Caco-2 cell line. Moreover, the ethanol extract of IO increased SOD1, CAT and KI67 mRNA expression and decreased ROS production in the BJ cell line. Alternately, PBS, ethanol and methanol extracts of IO decreased ROS production and SOD1, CAT and KI67 mRNA expression in Caco-2. The present study revealed, for the first time that IO extract acts in different way in normal and cancerous cell culture. Moreover cytotoxic mechanism of action can be partially ROS-dependent.
ISSN:1359-5113
1873-3298
DOI:10.1016/j.procbio.2018.07.015