A selective fluorescence probe for H^sub 2^S from biothiols with a significant regioselective turn-on response and its application for H^sub 2^S detection in living cells and in living Caenorhabditis elegans

A selective probe with a significant regioselective turn-on response, CP-1, has been developed for detection of H2S from biothiols. CP-1 features superb selectivity to H2S, a wide pH range and low cytotoxicity. CP-1 has over 10000-fold selectivity for H2S from biothiols such as GSH. With the actual...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2018-12, Vol.B276, p.456
Main Authors: Yang, Bing, Su, Mi-Mi, Xue, Yun-Sheng, He, Zhen-Xiang, Xu, Chen, Zhu, Hai-Liang
Format: Article
Language:English
Subjects:
Cells
Cells (biology)
Chemical synthesis
Fluorescence
Fluorescent indicators
Hydrogen sulfide
Medical imaging
Nematodes
NMR
Nuclear magnetic resonance
Organic chemicals
Regioselectivity
Selectivity
Sensors
Sodium sulfide
Toxicity
Urine
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Summary:A selective probe with a significant regioselective turn-on response, CP-1, has been developed for detection of H2S from biothiols. CP-1 features superb selectivity to H2S, a wide pH range and low cytotoxicity. CP-1 has over 10000-fold selectivity for H2S from biothiols such as GSH. With the actual detected concentration low to 50 nM, CP-1 could provide a set of sectionalized linear standard curves to cover a 0 to 275 μM detecting range accurately. NMR and HRMS spectra confirmed that the detection process was a regioselective reaction. Furthermore, mono-protected compound FL-1 has been synthesized and tested for Na2S and biothiols. All test results demonstrate that the detection can only occur in one side of CP-1. With high sensitivity and selectivity, CP-1 could be further used in rat urine samples system. Moreover, CP-1 has been applied in the imaging of exogenous and endogenous H2S in living cells and in living Caenorhabditis elegans.
ISSN:0925-4005
1873-3077