High-energy phosphate metabolism and creatine kinases in failing hearts: A new porcine model

BACKGROUND: This study aimed to create a pig model of heart failure secondary to severe aortic stenosis and to examine the relationship between the alterations in myocardial high-energy phosphate (HEP) metabolism and protein expression of creatine kinase (CK) isoforms. METHODS AND RESULTS: Sixteen p...

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Published in:Circulation (New York, N.Y.) N.Y.), 2001-03, Vol.103 (11), p.1570
Main Authors: Ye, Yun, Gong, Guangrong, Ochiai, Koichi, Liu, Jingbo, Zhang, Jianyi
Format: Article
Language:English
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Summary:BACKGROUND: This study aimed to create a pig model of heart failure secondary to severe aortic stenosis and to examine the relationship between the alterations in myocardial high-energy phosphate (HEP) metabolism and protein expression of creatine kinase (CK) isoforms. METHODS AND RESULTS: Sixteen pigs with left ventricular hypertrophy (LVH) secondary to ascending aortic banding and 10 normal pigs (N) were studied. Myocardial protein levels of CK isoforms (Western blot), HEP levels, and CK kinetics ((31)P MR spectroscopy) were measured under basal conditions. Nine of the 16 animals with LVH developed congestive heart failure (CHF), as evidenced by ascites (100 to 2000 mL). LV weight/body weight ratio (g/kg) was 2.18+/-0.15 in N hearts, 3.04+/-0.14 in hearts with LVH (P
ISSN:0009-7322
1524-4539